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Gene Symbol	Full name	Phenotypes	Entrez_id	PMID	Experimental evidences	Sample	Regulation
NKX2-3	NK2 homeobox 3	UC	159296	20957151, 21072187, 20165982	qPCR, RT-PCR, Genotyping, Western blot analysis, Ingenuity Pathway analysis, Microarray and data analysis, Immunofluorescence assay
NLRP3	NLR family pyrin domain containing 3	UC, CD	114548	19784369, 20385749, 30583612, 29102545, 28523573, 27966619, 20182451	SNP genotyping, qPCR, ELISA	Mice and Human
NOD2	Nucleotide binding oligomerization domain containing 2	IBD, CD	64127	25886994, 28099495, 25991924	miRNA microarray, RT-qPCR, SNP analysis	Human
NOD1	Nucleotide binding oligomerization domain containing 1	IBD	10392	21729873, 24155895	FACS analysis, RT-PCR and Microarray technology	Zebrafish
ATG16L1	Autophagy related 16 like 1	IBD, CD, UC	55054	28130498, 17435756, 20637199	Genotyping, IgA measurement, Western blotting, Histopathological analysis, RNA expression analysis, Immunofluorescence and Immunohistochemistry, RT-qPCR, SNP analysis, FACS and Microscopy	Human, Mice
TNF	Tumor necrosis factor	IBD, UC, CD	7124	22848538, 27895399, 12671897	Fish, qPCR, Histopathology, ELISA, Cytokine staining, RT-PCR and Western blotting	mice and Human
RIPK2	Receptor interacting serine/threonine kinase 2	IBD, CD	8767	26320862	Immunoblotting, qPCR and Flow cytometry	Human
TLR2	Toll like receptor 4	CD, UC, IBD	7097	28388655, 22434667, 19950179	Genotyping, SNP analysis, qPCR and FACS	Mice and Bacteria
TLR4	Toll like receptor 3	UC, CD, IBD	7099	29913461, 19664207, 17538633	qPCR and Western blotting, RFLP	Human
IL23R	Interleukin 23 receptor	IBD, UC, CD	149233	17447842, 17786191, 17030949	SNP analysis, FRET, qPCR, ELISA and FACS	Human, Mice
IRGM	Immunity related GTPase M	CD, UC, IBD	345611	20106866	Microarray expression analysis, qPCR, SNP analysis	Human
TLR3	Toll like receptor 3	IBD, UC	7098	28099447	1D SDS-PAGE, 2D Gel analysis and Gene expression
GAPDH	Glyceraldehyde-3-phosphate dehydrogenase	IBD, CD	2597	27812135, 19907652	(GWAS) RT-qPCR, Immunoblotting, ELISA, BRET analyses, Immunoprecipitation experiments, Yeast two-hybrid screening, SNP genotyping, siRNAs and NF-kappaB reporter assays, Western blotting with anti-MYC and anti-GFP antibodies
PTPN2	Protein tyrosine phosphatase non-receptor type 2	IBD, CD, UC	5771	22457781	(GWAS) PCR, DNA extraction, FRET and SNP genotyping, In silico analysis of transcription factor binding sites, Haplotype analysis	Human
MUC2	Mucin 2, oligomeric mucus/gel-forming	IBD, UC, CD	4583	27490946	Immunochip, High throughput sequencing
PIGR	Polymeric immunoglobulin receptor	IBD, CD, UC	5284	22491177	RT-qPCR, Tissue histology and Immunofluorescence microscopy		Down
BAHD1	Bromo adjacent homology domain containing 1	UC	22893	26183847	Immunohistochemistry, RT-qPCR, Western blot, ELISA, DSS induced colitic mice	Human, C57BL/6 Mice	Down
FFAR2	Free fatty acid receptor 2	IBD	2867	27667443	Immunohistochemistry, RT-qPCR, Western blotting		Down
FFAR3	Free fatty acid receptor 3	IBD	2865	27667443	Immunohistochemistry, RT-qPCR, Western blotting		Down
NCF2	Neutrophil cytosolic factor 2	UC	4688	21900546	Western blot, Cell culture and Immunoprecipitation, RT-PCR, Cell adhesion assay, NCF2 genotyping, SNP analysis and Genotyping, Subphenotype analysis, Apoptosis analysis		Up
RAC2	Rac family small GTPase 2	IBD, UC	5880	21900546	RT-PCR, Apoptosis analysis, SNP analysis and genotyping, Cell adhesion assay, Subphenotype analysis, Constructs, Western blot, Cell culture and Immunoprecipitation		Up
NCF4	Neutrophil cytosolic factor 4	CD	4689	21900546, 26289090	SNP genotyping and analysis, Subphenotype analysis, RT-PCR		Up
SEL1L	SEL1L adaptor subunit of ERAD E3 ubiquitin ligase	UC	6400	26551274	Western blot, Knockdown, RT-qPCR, Immunohistochemistry		Down
GLI1	GLI family zinc finger 1	UC	2735	19071955	RT-qPCR, SNP genotyping, Gene expression by microarray, NanoDrop ND-1000 spectrophotometer, Induction of DSS-induced colitis and Histological analysis, Immunohistochemistry, GLI1 protein mutagenesis, Luciferase assay and Immunofluorescence, Cytokine expre	Gli1+/lacZ Mice	Down
PTCH1	Patched 1	UC	5727	16733713	DNA extraction, SNP genotyping	Human	Down
IHH	Indian hedgehog signaling molecule	CD	3549	19071955, 20206176, 18479261	ELISA, DAVID, RT-qPCR	C57BL/6 mice	Up
AVIL	Advillin	IBD	10677	12034507	PCR, Mapping of AVIL by Fish, SNP analysis, Lightcycler analysis of AVIL mRNA expression, RT-PCR	Human
REL	REL Proto-Oncogene, NF-KB Subunit	UC	5966	21425313	(GWAS),Genotyping,and,SNP,selection,Allelic,association,analysis,Insilico,analysis,,GRAIL,analysis,eQTL,analysis,Principal,componenet,analysis	Human
ABCA1	ATP binding cassette subfamily A member 1	IBD	19	33609028	Western blot, Immunohistochemistry,Decreased mRNA expression of the LXR target genes ABCA1 and FAS was shown	IL-10-/- mice,Human
NR1H2	Nuclear receptor subfamily 1 group H member 2	UC,CD	7376	21245992	SNP genotyping, DNA extraction, Haplotype analysis	Human
PPARG	Peroxisome proliferator activated receptor gamma	UC,CD	5468	20591056, 26462850	RT-PCR, Gel electrophoresis,Total RNA extraction,qRT-PCR,Immunofluorescence,Immunohistochemistry,Bacterial culture,Cell culture	Human
TCF7L2	Transcription factor 7 like 2	UC	6934	19221600	SNP, Matrix assisted laser desorption/ionization time-of-flight (MALDI-TOF) based mass spectrometry (MS), RT-PCR, EMSA, Histological analysis, Promoter analysis, Sequencing of TCF-4 promoter and gene region, Genotyping TaqMan technology	Mice	Up
SLC22A4	Solute carrier family 22 member 4	CD	6583	19581171	SNP genotyping, PCR, Gene ontology	Human cohort study	Up
ICAM1	Intercellular adhesion molecule 1	IBD, UC, CD	3383	19212205, 18647548	Genotyping, SNP analysis, PCR, PCR-RFLP	Human
IL18RAP	Interleukin 18 receptor accessory protein	IBD, UC, CD	8807	19103669, 20176734, 18439550	SNP analysis, Western blotting	Human
JAK2	Janus kinase 2	IBD, UC, CD	3717	22065112	Genotyping,Real-time PCR	Mice, Human	Down
DEFA5	Defensin alpha 5	CD	1670	18305068	RT-PCR, Immunochemical staining		Low
APAF1	Apoptotic peptidase activating factor 1	UC	317	20113505	Immunohistochemistry,Immunoblotting,Gel electrophoresis,SDS-PAGE,Immunoprecipitation,
IL4	Interleukin 4	UC	3565	8964392	ELISA, RNA extraction and preparation of complementary DNA probes, Northern blot analysis
XBP1	X-box binding protein 1	IBD	7494	18775308	EM, qPCR, Microarray analysis, Immunohistochemistry, PAGE	Mice
CLEC7A	C-type lectin domain containing 7A	UC	64581	22674328	qPCR, Dapi, Immunofluorescent staining, Immunohistochemistry	Mice
ERBIN	Erbb2 interacting protein	CD	55914	19266573	Genotyping, RT-PCR, Western blot, Immunoprecipitation and Immunoblotting	Human
SLC15A1	Solute carrier family 15 member 1	IBD	6564	19462432	Genotyping, Dual-Luciferase Reporter Assay, MALDI-TOF mass spectrometry	Male mice
MUC19	Mucin 19, Oligomeric	IBD, UC, CD	283463	23128233, 19714762, 21152001	(GWAS) SNP genotyping, MALDI TOF	Human
CYLD	CYLD lysine 63 deubiquitinase	IBD, CD	1540	24092863	SNP analysis, siRNA transfection, PCR, Western blotting, Confocal microscopy
NOS2	Nitric oxide synthase 2	IBD, UC, CD	4843	24430113	SNP analysis and Genotyping, Immunohistochemistry
MTOR	Mechanistic target of rapamycin kinase	UC	2475	30132862	FITC, Myeloid-Derived Suppressor cells (MDSCs) differentiation assay, Flow cytometry analysis, DSS-induced experimental colitis in mice, ROS detection, Real-time PCR	Mice
ECM1	Extracellular matrix protein 1	UC	1893	28699600	RFLP-PCR, Genotyping	Human
BCL2	BCL2 apoptosis regulator	IBD, CD	596	30228896	FITC, FACS, RT-PCR, Fcal-ELISA
BAX	BCL2 associated X, Apoptosis regulator	UC, IBD, CD	581	30228896	FITC, FACS, RT-PCR, Fcal- ELISA
TLR10	Toll like receptor 10	IBD, CD	81793	21716313	qPCR, FRET, Genotyping, Taqman assay
TLR8	Toll like receptor 8	IBD	51311	18942751, 26486764	Genotyping, SNP analysis	Human cohort study
TLR7	Toll like receptor 7	IBD	51284	27084119	DSS treatment, qPCR, Microarray analysis, BlastN, Genotyping, Immunohistochemistry	Mice
TLR9	Toll like receptor 9	CD	54106	22893852, 17914947	(GWAS) Genotyping, RT-PCR, PCR-RFLP	Mice	Low
IL17A	Interleukin 17A	CD, UC	3605	26072972	qPCR, Immunohistochemistry, ELISA, FACS Caliber Flow cytometry	Human	Up
IL23A	Interleukin 23 subunit alpha	IBD, CD, UC	51561	20228197	qPCR, Cytokine ELISA, Chip, Flow cytometry	Mice	Down
MYD88	MYD88 innate immune signal transduction adaptor	IBD, CD, UC	4615	20433840	qPCR, Dapi, Immunofluorescent staining, Flow cytometry	Mice
CARD9	Caspase recruitment domain family member 9	IBD, UC, CD	64170	26488816, 29073062, 27158904	qPCR, In vivo injection of heat-killed C.albicans in PBS, Ex vivo splenic stimulations and intracellular phosphoprotein staining, FACS, Bead based-ELISA, Western blotting, Immunoprecipitation	Mice Strain -Trim62 and WT
CD4	CD4 molecule	IBD, UC, CD	920	26589955	Immunofluorescence, Suppression assay, FACS Aria cell sorter	Human
IL6	Interleukin 6	IBD, UC, CD	3569	19550417, 18799360, 22539302	Immunofluorescence microscopy, Western blotting, Flow cytometry, ELISA assay, qPCR, Genotype, MALDI-TOF/MS	Human cohort study
TLR5	Toll like receptor 5	CD	7100	16439468	SDS-PAGE immunoblotting, ELISA, Genotyping, Human cytokine array, Infection of adenoviruses, Immunofluorescence staining, PCR, Immunoprecipitation assay, Luciferase reporter assay	Human, Mice
DAP	Death associated protein	UC	1611	21297633	(GWAS) SNP analysis, In silico analysis	Meta analysis, Human cohort
PTPN22	Protein tyrosine phosphatase non-receptor type 22	UC	26191	23456301, 15986374, 16185327, 16391555, 16164701	Genotyping, RFLP-PCR, Serum level determination by immuno-nephelometry with Behring Nephelometer Analyzer II (BNII), mRNA expression analysis	Human
LRRK2	Leucine rich repeat kinase 2	CD	120892	29321258, 22430149	(GWAS) Exome sequencing, Human exome bead chip genotyping, Immunochip-base study, Immunoblot, Western blot, SDS-PAGE, Flow cytometry, GTP hydrolysis assay, TLC	Human, Mice

图文展示

Gene symbol	Full name	Phenotypes	Entrez_id	PMID	Experimental evidences	Sample	Up OR Down
TNFSF15	TNF superfamily member 15	CD	9966	19124533, 24835165, 24783249, 18422820, 18338776, 25028192	SNP genotyping, Kaplan meier method, ELISA	Human cohort study
IL12B	Interleukin 12B	IBD	3593	22479607, 23573954, 15483662	(GWAS) SNP analysis, PCR, FRET, Insilico analysis	Human cohort study
IBD5	Inflammatory bowel disease 5	CD	50941	18521914, 16642031	Fast qPCR, SNP analysis, Gene-gene Interaction analysis	Human cohort study	Up
IL18	Interleukin 18	IBD, UC, CD	3606	26229413, 12572878, 14738461	Meta-analysis by database searches, Cytokine assays by Sandwich ELISA, Histological Examination, Flow cytometry, qPCR	Human cohort, Mice
NLRP6	NLR family pyrin domain containing 6	CD	171389	21593405, 20403135	RT-PCR, DSS-treated mice, ELISA, Immunohistochemistry, Microarray and Gene ontology analysis	Mice
NLRP1	NLR family pyrin domain containing 1	IBD, CD	22861	25725098	ELISA, Generation of chimeric mice by bone marrow reconstitution, Histopathology evaluation, Polyp analysis, Human metadata analysis	Mice Nlrp1b_/_ and Asc_/_
TLR1	Toll like receptor 1	CD	7096	17595233	RFLP-PCR genotype, Serological analyses, ELISA, Multivariate genotype-phenotype-serotype associations in crohn'S disease	Human cohort
TLR6	Toll like receptor 6	CD	10333	24670426	DSS-induced colitis model, Immunoblot, Immunofluorescence staining, qPCR, Ex vivo T-cell Stimulation, PBMC culture, FACS canto II Flow cytometer, Fcap array	Human, Mice
TNFAIP3	TNF alpha induced protein 3	UC, CD	7128	25956706	Multivariate analysis of biomarker expression in colonic mucosa, Taqman gold RT-PCR, mRNA transcripts analyzed by RT-qPCR	Human
CD14	CD14 molecule	CD, UC	929	15764151, 15655821	RFLP-PCR, Genotyping	Human cohort
NLRP12	NLR family pyrin domain containing 12	IBD, CD	91662	22094258, 31169706, 20403135	In situ intestinal proliferation assay, In vitro signaling assays, Multiplex ELISA, qPCR, Induction of DSS induced colitis, Histopathological analysis, Bone marrow chimeras, Isolation of lamina propria cells, NF-KappaB activity assay	Mice	Down
CIITA	Class II MHC transactivator	IBD, CD	4261	17043423	DSS-induced colitis, Oxazolone-induced colitis, Cytokine ELISA, Histopathological analysis	Mice C57BL/6 (B6)
XIAP	X-Linked inhibitor of apoptosis	IBD, CD	331	28404814	Antibodies and Western blotting, NOD signaling defects and cell death susceptibility, CRISPR/SPCAS9 knockout of XIAP and stable cell line generation, qPCR, TNF and LPS cytotoxicity assays, Propidium iodide influx assays, Acquisition of patient PBMCs and F	XIAP/ mice
APC	APC regulator of WNT signaling pathway	UC, IBD	324	9744312, 17192888	PCR, PAGE, Ileorectal anastomosis (IRA), in vitro synthe-sized protein (IVSP)
DLG5	Discs large MAGUK scaffold protein 5	CD, IBD, UC	9231	16494592, 15107852	Genotyping for DLG5 variants, PCR, FRET probes, Measurement of gastro-duodenal and intestinal permeability, Genotyping for Card15 variants, SNPs genotyping analysis, Microsatellite typing	Human
DMBT1	Deleted in malignant brain tumors 1	CD, IBD	1755	17983803, 24223725	siRNA transfection, qPCR, Western blot, Electrophoretic mobility shift, Luciferase assays, RT-qPCR, Immunohistochemistry, mRNA In situ hybridization, Haplotype association	Human, Mice	DMBT SNP�s; Up
TNFRSF17	TNF receptor superfamily member 17	IBD	608	16318629	SNP genotyping, Tailed primer PCR, Microsatellite markers analysis	Human
KLF6	Kruppel like factor 6	IBD	1316	26838049	Western blot, RT-PCR, Immunofluorescent confocal staining	Mice	Up
FUT3	Fucosyl transferase 3 (Lewis Blood Group)	UC	2525	26766790	SNP genotyping, Immunohistochemistry	Human	FUT3 polymorphisms overrepresented in UC patients vs controls
FUT2	Fucosyl transferase 2	CD, IBD	2524	24720527, 20570966, 24828903, 22068912, 23075394, 23002346	Genotype analysis By multiplex SNAP assay of Abi, Multiplex PCR, SNP tested in replication of FUT loci by taqman minor groove binder chemistry, Taqman SNP genotyping assays	Human cohort
IBD2	Inflammatory bowel disease 2	IBD, UC	3378	12439891	Genotyping, PCR, Electrophoretic mobility shift assay (EMSA), Criterion blotter, Chemiluminescent nucleic acid detection module (Pierce Biotechnology, Rockford, Il, Usa)	Human
IRF5	Interferon regulatory factor 5	IBD, UC, CD	3663	20127100, 22257839, 3809990, 25564941	Bisulfite pyrosequencing, CpG density calculation	Human
NLRX1	NLR family member X1	IBD	79671	29535731	Experimental model of colitis, Histopathology, Olympus microscope, qPCR, RNA sequencing	Mice C57BL/6 wild-type (WT), Nlrx1_/_, Nlrx1fl/fl, and Nlrx1fl/fl, Villin Cre+ mice	Up
BTNL2	Butyrophilin like 2	UC, IBD, CD	56244	17610417, 19659809, 17237401, 25671699	Direct genome sequencing, PCR-RFLP, Association study of HLA_DRB1	Human cohort
MST1	Macrophage stimulating 1	IBD, UC, CD	4485	19657358, 19079170, 22087277, 18438406, 21931648	(GWAS) Genotyping by Taqman assays, Fast qPCR, SNP analysis, SNP Selection from hapmap database, Mass spectrometry analysis, Allelic tests, RT-PCR, SNP genotyping with infinium I assay (Illumina), Plink, MALDI-TOF mass array platform	Human cohort
CUL2	Cullin 2	IBD, CD	8453	23639549, 30228239	(GWAS) SNP Test, RT-PCR, Gtex SNP(rs12248333), IBD-associated SNP (rs34779708), EQTL analysis, Haplotype analyses, Selection analyses, Credible set analysis, E. Histolytica culture, Cre-luciferase reporter assays, Crem expression In Q223R mice, Caspase-3	SV129/Bl6.CREM_/_ Mice
FOXP3	Forkhead box P3	IBD, UC	50943	27178149, 28337881	(GWAS) qPCR, mRNA expression, Measurement of serum retinol levels by HPLC, Immunohistochemistry, Histopathology	Human
DUOX2	Dual oxidase 2	IBD, CD, UC	50506	26301257, 25003194, 17454855	Epifluorescence light microscope (Leica microsystems, Buffalo Grove, Il), Protein isolation, Western blotting, Ros assays, Flow cytometry, Immunofluorescence, Virulence assay, Cytoscape.V3.0.2, Immunohistochemistry, Olympus Bx51 light microscope, RT-PCR	Huamn
IL2	Interleukin 2	UC, IBD, CD	3558	21648020, 19201773, 19471255, 8100205, 12591057	SNP analysis, Taqman technology for genotyping, Mass spectrometric analysis, ELISA, ADCC assay, Imminofluorescence staining, Flow cytometry	Human
MYC	MYC proto-oncogene, BHLH transcription factor	IBD, CD	4609	1612482	RNA preparation, Slot blot hybridization, Northern hybridization, in vitro translation, Immunohistochemical studies, expression of C-Myc proto-oncogene	Human
IL22	Interleukin 22	UC, CD	50616	16537974, 26994530	RT-PCR, qPCR, Signal-transduction experiments, Gel electrophoresis, Immunoblotting, ELISA, Wounding assay, Cell proliferation assay, Apoptosis assays, DSS induced colitis model	Mice, Human	Up
HDAC6	Histone deacetylase 6	IBD	10013	27827303	Light microscope, DSS induced colitis, Histology, Mucin staining, Esterase staining, Gut cell isolation, Flow cytometry, RT-PCR analysis, Isolation of human polymorphonuclear leukocytes, HPLC, MS	Female C57BL/6 mice
DEFB1	Defensin beta 1	CD	1672	17921115, 22522766	RT-PCR, Fluorescent microscope, qPCR, DEFB1 SNP genotyping	Human cohort
BIRC3	Baculoviral IAP repeat containing 3	UC	330	23388545	RNA extraction, Microarray analysis, Gene filtering and sample classification were performed using Dchip software, qPCR, Immunohistochemistry	Human
RNF186	Ring finger protein 186	UC	54546	24068945, 27503255	(GWAS) DNA preparation and pooling, PCR, Target selection and design, Sequencing and variant discovery, Genotyping, Validation and follow-up genotyping using sequenom mass array Iplex200, Network analyses, Exome sequencing, MALDI-TOF, Immunofluorescence,	Human cohort, Mice
CRP	C-Reactive protein	pIBD, CD, UC	1401	16497618, 25636121, 18566104	Genotyping, PCR-RFLP, SNP selection, DNA extraction from venous whole blood by salting out technique, Response to anti�TNF_ monoclonal antibody	Human cohort
IL2RA	Interleukin 2 receptor subunit alpha	UC, IBD	3559	23972291, 19471255	Genotyping using primer extension chemistry and Mass spectrometric analysis(Iplex assay, Sequenom, San Diego, Ca), Multiplex PCR, MALDI-TOF Mass spectrometry, Genotyping by Taqman assays from applied biosystems
CD40	CD40 molecule	IBD, CD, UC	958	15591506, 10566730, 16149136	Elucidation of the sequence of rat CD40 mRNA, Immunohistochemistry, Fluorescein (FITC), Immunofluorescence microscopy, Transfection of antisense oligonucleotides(ODNS) in vitro, Western blot analysis, RT-PCR analysis, Colitis induction, Flow cytometric an	Rat
MEFV	MEFV innate Immuity regulator, Pyrin	CD	4210	22810105, 15667491	(GWAS) Genotyping for The MEFV variants, PCR	Human
AIM2	Absent in melanoma 2	UC	9447	26313692, 26655906	Histopathological examination, Affymetrix microarrays, RT-qPCR, RNA isolation, RNA quantity and quality assessed using nanodrop Nd-1000 spectrophotometer, Microarray gene expression data analysed using limma, Immunohistochemistry, Western blot analysis, P	Human Cohort, Mice	Down
BIRC2	Baculoviral IAP repeat containing 2	UC	329	30005591, 28422189	(GWAS) Geo data collection and preprocessing, Network clustering by DPCLUSO, DEGs		UP
CCL5	C-C motif chemokine ligand 5	IBD, UC	6352	19639558, 11872088	qPCR, Immunocytochemistry, Proteomics, Histopathology, Microarray analysis, (LC/MS/MS), Microbial analysis by denaturing gradient gel electrophoresis (DGGE), Sequencing of DGGE bands	Mice IL10-KO and wildtype (WT)	Down
ATG7	Autophagy related 7	UC	10533	30669622, 19139628	(GWAS) Lysozyme staining, The role of the autophagy pathway in paneth cell abnormalities	Mice
AKT1	AKT Serine/Threonine kinase 1	IBD	207	28659381	Induction of colitis, Haematoxylin and Eosin staining, Immunohistochemistry, HCT-8 and Hela cells were used for transfection, Western blot, qPCR, Rt_ Profiler� PCR array data analysis web-based tool (Qiagen, Sa Biosciences, Usa), Immunoprecipitation, ELIS	Female C57BL/6 mice	Down
MPO	Myeloperoxidase	UC, IBD, CD	4353	18810778	Calprotectin ELISA, Radioimmunoassay, Histopathology
PSTPIP1	Proline-serine-threonine phosphatase interacting protein 1	IBD	9051	19731031	EDTA, DHPLC, PCR, PSTPIP1 Microsatellite identification and typing, Genomatix suite used for promoter sequences	Human

Gene Symbol	Full name	Phenotypes	Entrez_id	PMID	Experimental evidences	Sample	Up OR Down
PGLYRP1	Peptidoglycan recognition protein 1	IBD	8993	20709292, 23840689	Western blotting, PGRP-Deficient mice have higher susceptibility to DSS induced colitis, Flow cytometry, RT-qPCR, Cell isolation, Culture, Cytokine production, Amino acid sequence homology in mammalian PGLYRPS in the regions of non-synonymous polymorphism	Mice BALB/c
CD80	CD80 molecule	IBD, UC, CD	941	25595911, 14530338	Flow cytometry, Histopathology, Immunohistochemistry, RT-qPCR, Immunoassay, Induction of colitis and treatments, Cytokine ELISA	C57Bl6/J Mice, BALB/c mice
ICOSLG	Inducible T cell costimulator ligand	IBD, CD	23308	24837102, 20176734, 21072187	(GWAS) FACS, ELISA, Transfection of small interfering RNAs (siRNAs), Western blot, qPCR, Genotyping and association tests	Human, Mice
ITLN1	Intelectin 1	CD	55600	18587394	(GWAS) Haplotype patterns, Hapmap SNPs, EQTL analysis, SNP analysis
IL9	Interleukin 9	UC, IBD	3578	28652656, 25838986, 26377844	Flow cytometry based method utilizing magnetic microspheres, High-sensitive C-reactive protein (HSCRP), Fluorescence based assay, qPCR, Western blot analysis, RT-PCR, Immunofluorescence microscopy, TNBS colitis model, Immunofluorescence staining, ELISA	BALB/c and IL-9 deficient mice	up-regulation
IFNG	Interferon gamma	UC	3458	20848535, 25171510	(GWAS) IFN-assay By ELISA, Pyrosequencing, Hot-start PCR, Isolation of T cells	Human
IL13RA2	Interleukin 13 receptor subunit alpha 2	IBD	3598	22741617, 28612843, 20014020, 30663072	PCR-RFLP, SNP analysis, Immunohistochemistry	Balb/c mice, Human
ZNF365	Zinc finger protein 365	CD	22891	21257989, 20222910, 25489960	RNase minikit (Qiagen, Valencia, Ca), SNP selection and genotype, RT-PCR, Illumina total prep RNA amplification kit (protocol from Ambion, Austin, Tx), Nanodrop spectrophotometer, qPCR	Human
LAMB1	Laminin subunit beta 1	UC, IBD	3912	19915572	(GWAS) Picogreen assay, Sequenom Iplex assay, GWA genotyping, Replication genotyping, SNP analysis
CD68	CD68 molecule	CD	968	26802079	Bradford assay, Immunostaining, Immunohistochemistry, Immunofluorescence and Goblet cell count used as a secondary antibody, Western blot, FITC, RT-qPCR, Chromatin immunoprecipitation (Chip) assay	Human
HNRNPA1	Heterogeneous nuclear ribonucleoprotein A1	CD	3178	19349988	Immunoblot analysis, Chip, RT-PCR, DNA affinity binding assay, Retroviral and Lentiviral packaging and macrophage transduction, Immuno-precipitation, Nanoflol Liquid chromatography�tandem MS analysis, ELISA	Mice
KCNN4	Potassium calcium-activated channel subfamily N member 4	CD	3783	20407432	RT-PCR, qPCR, Immunohistochemistry, MALDI-TOF mass spectrometer, SNP identification and genotyping, Sequenom mass-array assay	Mice, Human
SIGIRR	Single Ig and TIR domain containing	UC	59307	21077278, 20130217	Immunoprecipitation (Chip) assays, Human IL-8 enzyme immune assay (EIA), FITC, qPCR, Immunohistochemistry assays, Flow cytometry, RNA interference, Transient transfection and NF-KappaB, Luciferase assay, Chromatin immunoprecipitation (Chip) assay, Gel shi	Mice	Down
ORMDL3	ORMDL Sphingolipid biosynthesis regulator 3	CD	94103	21072187, 27932194, 28333149, 20228799	(GWAS) SNP genotyping assay(Taqman�), qPCR, SNP analysis, DSS-induced colitis in mice	Human, Mice	Up
TOLLIP	Toll interacting protein	UC, CD	54472	18985539, 26462859	Luciferase assay, Electrophoretic mobility shift assay, Immunoblotting, qPCR, Immunofluorescence microscopy, Immuno-electron microscopy (Em), Indirect Immunofluorescence microscopy, ELISA	Female BALB/c mice	Up
OLFM4	Olfactomedin 4	IBD	10562	20941359, 30137272	(GWAS) Microarray analysis of RNA expression, RT-PCR, Data analysis, NCBI gene expression omnibus (Geo), qPCR, Library preparation and Next-generation sequencing with the SeqCap Epi CpGiant enrichment kit	Human	Up
CCL21	C-C motif chemokine ligand 21	UC, CD	6366	24841666, 16118351, 26520877	Immunohistochemistry, DSS induced colitis, RT-PCR, Western blotting, Dapi, Immunohistochemistry staining in the intestinal mucosa of mice	Balb/c mice	Up
ATG16L2	Autophagy related 16 Like 2	CD	89849	27611316, 23850713	(GWAS) Genotyped using taqman SNP genotyping assay, Relationship between genotypes and phenotypes, qPCR, Bioinformatics analysis, mRNA expression of ATG16L2, Imputation	Human
SLC25A20	Solute carrier family 25 member 20	IBD, UC	788	26874350	Bacterial preparations, RNA isolation, Microarrays, qPCR, RT-PCR	Human	Up
SLC15A4	Solute carrier family 15 member 4	IBD, UC	121260	21277849	Immunohistochemical staining, Quantification of cytokines using a bead cytometric bead array mouse inflammation kit, Assay for cathepsin activities, DSS induced colitis model, Bone marrow transplantation, Stimulation of mice with NOD ligands, Flow cytomet	Mice C57BL/6 Slc15a4-Deficient mice
NLRP7	NLR family Pyrin domain containing 7	UC, CD	199713	29211899	Whole-exome sequencing (Wes), Mapping, Pathogenicity prediction, PCR, Sanger Sequencing confirmation of NLRP7 variants, Genotyping	Human
GSDMB	Gasdermin B	UC, CD	55876	28154144, 26484354	Cloning, Expression, Protein purification, Crystallization, Data collection, Structure determination, Protein lipid overlay assay, in vitro caspase cleavage assay, SDS-PAGE, Nanodrop Nd-1000, RT-PCR, Gene expression analysis and Data preprocessing, Genoty	Human
EGFR	Epidermal growth factor receptor	UC	1956	24391216, 29904166	DSS-induced colitis, Western blot analysis, Multi-color flow cytometry, Myeloperoxidase (MPO) assay, Immunophenotyping, Colonic macrophage isolation, Isolation of spleen lymphocytes, qPCR, ELISA, Luciferase activity, SDS-PAGE, Immunohistochemistry, FITC,	Egfrfl/flLysM-Cre mice and UC Human
MAPK3	Mitogen-activated protein kinase 3	IBD, CD	5595	26636514	Bioinformatic analysis, Affymetrix microarray data, Identification of differentially expressed genes, Gene ontology and pathway enrichment analysis of DEGs using Kegg, Go and David, PPI Network and Module analysis, RT-qPCR analysis	Human, Rats
MAP3K8	Mitogen-activated protein kinase 8	IBD, UC	1326	26215868	Transfection of small interfering RNAs (SiRNAs) and Plasmids, Protein expression analysis, mRNA expression analysis, qPCR, RT-PCR, Genotyping, Primary human myeloid cells from IBD, Flow cytometry	Human
RNF31	Ring finger protein 31	IBD, UC	55072	31132297	Human cohort and microarray analysis, Evaluation of mouse colitis, Immunohistochemistry and Immunofluorescent hybridisation, Reagents and antibodies	Male BALB/c mice	Up
IL31RA	Interleukin 31 receptor A	IBD, UC	133396	17449633	RT-PCR, qPCR, SYBR green PCR kit, Signal transduction experiments, Gel electrophoresis, Immunoblotting, Immunofluorescence staining, ELISA, Cell proliferation and cell restitution assays, Murine cytomegalovirus infection in vivo	C57BL/6 wild type Mice	Up
IL21	Interleukin 21	UC	59067	21648020	SNP genotyping, qPCR	Human
IL15	Interleukin 15	UC, CD	3600	10725717, 26964669	FITC or Pe-conjugated anti-CD3, Flow cytometric analysis, Immunohistochemistry, IL-15 production by LPMC, T cell activation by IL-15, Measurement of cytokines, ELISA, In situ expression of IL-15 In intestinal tissue	Human
ATG4D	Autophagy related 4D cysteine peptidase	CD	84971	22261526	(GWAS) Genotyping, RFLP	Human
LACC1	Laccase domain containing 1	CD	144811	27959965, 27098602	Western blot, Transfection, Bradford colorimetric assay, SDS PAGE, Immunofluorescence staining, Antibodies used for immunofluorescence and western blot, Laccase assay, qPCR, FACS flow cytometry, In silico analyses, SNP genotype	Human	Up
FASLG	Fas ligand	CD, IBD, UC	356	22860894, 26374830, 9771405	Genotyping, Immunohistochemistry, Assessment of immunohistochemical staining, RT-PCR, In situ hybridization, Flow cytometry	Human, Mouse
IL7	Interleukin 7	IBD, CD	3574	23057802, 18097039	Induction of colitis and antibody treatments, Pathology and Immunohistochemistry, Serum cytokines and anti-Hb antibodies, Quantitative RT-PCR analyses, ELISA, Flow cytometry	Mice Mdr1a_/_, Rag2_/_ mice, C57BL/6-Ly5.2 mice, C57BL/6-Ly5.1 mice and C57BL/6-Ly5.2-RAG-2-deficient (RAG-2/) mice
ATG4A	Autophagy related 4A cysteine peptidase	CD	115201	22261526, 21122541	m	Human
EGF	Epidermal growth factor	IBD, CD	1950	20969541	Sandwich ELISA	Human
CD44	CD44 molecule (Indian Blood Group)	IBD, UC	960	9863483, 10378517	Antibody characterization ELISA, Immunohistological detection of CD44 v6 expression in ulcerative colitis and crohn�S disease, Hewlett-packard ELISA reader, Determination of soluble CD44 v6 96 well immunoassay plates (Immunoplate I), Staining with antibod	Mice BALB/c
IL13	Interleukin 13	IBD, UC, CD	3596	22741617, 23746199	Genotyping determined by PCR-RFLP
CD19	CD19 molecule	CD	930	25914477	Demditec Epstein-Barr Virus VCA IgG antibody ELISA test kit (Demeditec diagnostics Gmbh, Kiel, Germany), Anti-EBV IgG antibodies	Human
CDKN2A	Cyclin dependent kinase inhibitor 2A	UC	1029	29063720	(GWAS) Genotyping using illumina omniexpress, Omni express exome and Omni1-quad arrays, 7900Ht fast qPCR system, UCSC genome bioinformatics and Genecards were utilized, SNP was performed using the encode, Genotype tissue expression (Gtex)29	Human	Up
THY1	Thy-1 cell surface antigen	UC	7070	22826509, 22202458	(GWAS) Qiaamp tissue DNA preparation kit (Qiagen), Three-layer genome wide scans for differential gene expression, Discordant for UC, Expression profiling (mRNA, Layer I), Human methylation27 beadchips (Iscan System, Illumina), Validation of differential	Human	Up
IFNA1	Interferon alpha 1	CD	3439	7861027	Dot blot analysis	Human	Up
CD86	CD86 molecule	IBD, CD, UC	942	18069758, 9438323	PCR, Immunocytometry, Flow cytometric analysis	Human	Up
CSF2	Colony stimulating factor 2	UC, CD	1437	29434451, 19230854	ELISA	Human	Up
TP53	Tumor protein P53	UC, CD	7157	20110711, 17676397	SNP genotyping, Immunohistochemistry staining	Human	Up
CEACAM6	CEA cell adhesion molecule 6	CD	4680	19737864	Histological analyses, Confocal microscopy	Mice	Up
CXCL8	C-X-C motif chemokine ligand 8	CD	3576	15214047	Immunohistochemical staining, ELISA	Human	Down

Gene Symbol	Full name	Phenotypes	Entrez_id	PMID	Experimental evidences	Sample	Up OR Down
ATG5	Autophagy related 5	CD	9474	27096233	SNP genotyping	Human	Down
NFKB1	Nuclear factor Kappa B subunit 1	CD	4790	18092345, 17049230, 24945726	Chemiluminescence, Western blot, SNP genotyping, RT-qPCR, Immunohistochemical staining	Human	Up
LRP6	LDL receptor related protein 6	CD	4040	22393312	SNP genotyping, qPCR, Immunohistochemistry	Human	Down
STAT1	Signal transducer and activator of transcription 1	UC	6772	27220814, 23128233, 12171960	Flow cytometry FACS aria cytometer, LPMCs were cultured in 200 ml complete medium in 96-well plates, qPCR, Multiplex ELISA, Staining of cells for phenotyping and counting, Plasmacytoid dendritic cell isolation kit, PBMCs were isolated by density-gradient	Human	Up
IL10	Interleukin 10	CD	3586	16043989, 29023267, 24128120, 24216686	Abi Prism 3100 automatic sequencer (applied biosystems, Foster City, Calif), Genemapper V3.0 (applied biosystems), SNP genotyping, qPCR, FACS canto II Flow cytometer, Analyzed using flowjo, Ingenuity pathway analysis, RNA in situ hybridization, Immunochem	Mice(Il10-/- and Il10rb-/- mice)
DEFA6	Defensin alpha 6	CD	1671	26891258, 19549264, 18305068	qPCR, Luciferase assays, Chromatin Immunoprecipitation assay, Immunohistochemistry, RT-PCR validation of selected Transcripts, Variability in gene expression profiles of two independent sets of UC RNA samples, Chromosomal locations of differentially expre	Human	Up
TREM1	Triggering receptor expressed on myeloid cells 1	IBD	54210	22721842, 21910175, 21763322, 18975078	ELISA, Westergren method, Trem-1 SNP and genotyping	Human	Up
CX3CL1	C-X3-C motif chemokine ligand 1	IBD	6376	17241867, 17285595	Taqman technology, qPCR, Stimulation of himec with cytokines, Leukocytes and CD40L, Flow cytometric using a coulter epics Xl flow cytometer (Beckman Coulter Inc., Fullerton, Ca), Characterization of himec and PBMC, Immunohistochemistry, Western blotting,	Human	Up
HORMAD2	HORMA domain containing 2	CD	150280	19915574	(GWAS) Selection of SNPS genotyping	Human
IBD4	Inflammatory bowel disease 4	CD	50608	10747815	Genotyping by Abi prism linkage mapping set version 2, IBD susceptibility genes	Human
MOCOS	Molybdenum cofactor sulfurase	CD	55034	19500084	Known SNPS in (Xdh, Mocos and Aox1) selected using the applied biosystems, Taqman SNP genotyping assays, qPCR, Sequencing by Beckman Coulter dye terminator sequencing kit	Human
MIF	Macrophage migration inhibitory factor	IBD, UC, CD	4282	24687413, 17206642, 18452219, 18023095, 12109441	RFLP-PCR, Genotyping, ELISA, RT-qPCR, Spectrophotometry, Western blot analysis, Cytokine assays, Flow cytometry	Human
MME	Membrane metallo-endopeptidase	IBD	4311	23827863	RNA isolation and qPCR, High-capacity cDNA reverse transcription kit, DNA isolation and genotyping	Human
TNFRSF25	TNF receptor superfamily member 25	IBD, CD	8718	27001939, 26072972	FACS calibur flow cytometer (Bd) using cell quest analysis software (Bd), RNA isolation, qPCR, Primer-blast software, ELISA	Human	Up
VWF	Von willebrand factor	IBD, UC	7450	14966927	ELISA, Radioimmunoassay, Platelet count (Pc), Immunoassay	Human
TFF3	Trefoil factor 3	UC	7033	15932366	Sigmoidoscopic and Histological scoring	Human	Down
CCR7	C-C motif chemokine receptor 7	CD	1236	16118351	Immunohistochemistry, RT-PCR analysis, Immunostaining	Human cohort
AHR	Aryl hydrocarbon receptor	IBD, CD	196	20878756, 26802080	Fluorescent immunohistochemistry, Histological staining, RNA isolation kit, RT-PCR, SYBR green qPCR supermix, Bead based immunoassay, Proliferating cell nuclear antigen assay	Male C57BL/6 and AhR _/_ mice	Up
HMOX1	Heme oxygenase 1	UC, IBD	3162	18460015, 16804400, 20509889	Genotype assessment, Taqman qPCR, Microsatellite instability, Western blot, Immunohistochemical staining, Genotyping	Human	Up
ITGB2	Integrin subunit Beta 2	IBD, UC, CD	3689	12139949, 17109102, 9438323	Immunofluorescent labeling, FITC, Flow cytometry analysis	Human
POU5F1	POU class 5 homeobox 1	IBD, UC	5460	25664710	DNA isolation and genotyping, Spectrophotometer, Illumina veracode microarray Chip, SNP genotypes
CSF1	Colony stimulating factor 1	IBD, UC	1435	11889070, 28273887	Radioimmunoassays and Immunoassays, Immunohistochemistry, ELISA	Human	Up
CCR5	C-C motif chemokine receptor 5 (Gene/Pseudogene)	IBD, CD, UC	1234	28273887	Western blot analysis, Flow cytometry, ELISA	Human	Up
VEGFA	Vascular endothelial growth factor A	IBD, UC, CD	7422	27270504, 16911686, 15113369, 10389696, 17879276, 29556626, 25076849, 17188371, 16954806	Immunohistochemistry and Image analyses, Immunostaining, ELISA, Immunoassay, Immunohistochemical studies of VEGF, SNPs analysis		Down
CFLAR	CASP8 and FADD like apoptosis regulator	UC, CD	8837	23371318, 16472594	Immunohistochemistry, IEC purity determination by RT-PCR, RT-qPCR, Antibodies, Immunoblotting, Chemiluminescence	Human	Up
IL17F	Interleukin 17F	UC, IBD	112744	17828618, 19263269, 18022867, 26558270	Genotyping of polymorphisms by PCR-SSCP method, One-tube multiplex PCR, PCR-RFLP
PTEN	Phosphatase and Tensin homolog	UC	5728	20067111, 23516074	Immunohistochemistry, Immunohistochemical detection, Western blot analysis, Tunel assay	female Balb/c mice
F9	Coagulation factor IX	CD	2158	29945876	DSS-Induced colitis model and tissue preparation, Immunoblotting, Myeloperoxidase activity assay, CXCL1 ELISA	C57BL/6J WT control mice (F9+/+), F9_/_ mice
IGF1	Insulin like growth factor 1	IBD, CD	3479	21287667, 14691340, 11914023, 19751734	Serum assays, ELISA method, Western immunoblotting, qPCR, Immunostaining	Human cohort
CASP3	Caspase 3	IBD, CD	836	24553140, 15479684	EBV-transformed B-cell lines, Taqman SNP genotyping assay, Immortalized macrophage progenitors, Cellular ATG16L1 cleavage assays, Image-based flow cytometry, Western blots and Caspase assays	Mice
S100A9	S100 calcium binding protein A9	IBD1, CD, UC	6280	25781366, 29115397, 28138990, 28735301	Measurement of fecal calprotectin by quantitative ELISA, Immunochromatographic technology, Quantitative fecal immunochemical test, Immunohistochemistry, Immunocytochemistry, Relationships between post operative endoscopic recurrence, Fcal and HSCRP levels	Human
FCGR3B	Fc fragment of IgG receptor IIIB	IBD, UC	2215	23917248, 23128233	(GWAS) Multiplex PCR, SNP genotype	Human
CD2	CD2 molecule	CD, UC	914	7680015	Immunohistological labeling, in vitro cell aggregation, Effect of blocking antibodies against surface adhesion molecules	Human
SRC	SRC Proto-oncogene, Non-receptor tyrosine kinase	UC	6714	8674385	Isolation of colonic epithelial cells and purification of RNA, Probes, Electroelution and Chromatography on an Elutip-D column, Northern Blotting of RNA, Slotblotting, Radioactively labeled probes	Human	Down
LEP	Leptin	IBD, UC, CD	3952	17700265, 18383234, 9586759, 15784704, 15012781, 18849144	ELISA, Radioimmunoassay of stored serum, Erythrocyte sedimentation rate, BMI, Serum albumin and C_reactive protein concentrations and white blood cell counts were determined	Human
PI3	Peptidase inhibitor 3	UC, IBD	5266	29084078, 17200145	RNA extraction and RT-qPCR assays, Immunohistochemical analysis, ELISA	Human
CA2	Carbonic anhydrase 2	UC	760	11956656, 27742763	ELISA	Human
BCAR1	BCAR1 scaffold protein, Cas family member	CD, PIBD	9564	23665963	(GWAS) ELISA, SNP analysis
NLRP10	NLR family Pyrin domain containing 10	CD	338322	20403135, 25153506	(GWAS) Haplotype tagging SNPS, Sub-phenotype analysis
TIRAP	TIR domain containing adaptor protein	IBD	114609	17538633, 29441063	Genotyping using the sequenom mass array system (all genotypes on collections IBD-1 To -5 were generated), Determining haplotypes
PSMD4	Proteasome 26S subunit, Non-ATPase 4	IBD, UC	5710	14621278, 10807895	Immunohistochemical staining, A lowering in the inflammatory blood parameters ESR, CRP andorosomucoid was demonstrated, Antisecretory Factor, Preparation of AF and test of AF potency, Histopathological examination of rectal biopsies	Human
FCGR3A	Fc fragment of IgG receptor III A	IBD, CD, UC	2214	23917248, 29333082, 23128233, 21091999	ELISA, PCR-RFLP, SNP genotyping, PCR-based real-time invader assay
ITGAM	Integrin subunit alpha M	CD, UC, IBD	3684	9473377, 9355789	SNP genotyping, Flow cytometry triple fluorescence analysis
PIK3CA	Phosphatidylinositol-4, 5-bisphosphate 3-kinase catalytic subunit alpha	UC	5290	21442372	ELISA, DSS-induced colitis, Stained with Hematoxylin and Eosin, Immunohistochemistry	Female Balb/c mice
HLA-DRA	Major histocompatibility complex, Class II, DR alpha	UC, CD	3105	23128233, 19122664, 12073071	(GWAS) EQTL SNPS, Network tools grail and dapple, SNP analysis, All downstream genotyping done by SNPlex and Taqman technologies
VIM	Vimentin	CD, IBD	7431	21699783, 18626977, 2124983	Co-Immunoprecipitation, Genotyping by Taqman, SNP analysis, Immunofluorescence, Luciferase assay, Western blots, Hematoxylin and Eosin staining, Immunohistochemistry	Human
CD28	CD28 molecule	IBD	940	14518246	Immunohistochemistry	Human
CDKN1A	Cyclin dependent kinase inhibitor 1A	IBD, UC, CD	1026	18507080, 24760188, 15060836	RT-qPCR, Illumina mRNA microarray platform was used for mRNA microarray assays, Transfection of miR-Mimic and Inhibitors, Western blotting, Cell cycle analysis by flow cytometry, Luciferase reporter assays	Human	Up
CCDC122	Coiled-coil domain containing 122	CD	160857	22984114	(GWAS) Genotyping using high-throughput sequenom mass array platform, SNP analysis	Human
FAS	Fas cell surface death receptor	UC, CD, IBD	355	15637757, 16049281, 16856205	PCR, SNP analysis, DHPLC, Haematoxylin and Eosin staining, Immunoreactivity scores, Immunohistochemistry, Flow cytometry	Human	Up
FASN	Fatty acid synthase	IBD, UC	2194	16753600	Immunohistochemistry	Human	Up
JUN	Jun Proto-oncogene, AP-1 transcription factor subunit	IBD	3725	8674385	Cytoplasmic, Nuclear, Tumor suppressor genes proto-oncogene probes were used, Electro-elution and Chromatography on an Elutip-D column, Slot blotting and Northern blotting of RNA	Human
DEFB4A	Defensin beta 4A	UC, IBD, CD	1673	19617917, 17387677, 18346318, 12519391	ELISA, Immunoelectron microscopy, Immunohistochemistry, qPCR, ImmunoFlow cytometry	Human
IRAK3	Interleukin 1 receptor associated kinase 3	IBD, UC	11213	17558906	Genotyping and SNP selection, Microsatellite marker PCR	Human

图文展示

Gene Symbol	Full name	Phenotypes	Entrez_id	PMID	Experimental evidences	Sample	Up OR Down
ITGB1	Integrin subunit beta 1	IBD, UC	3688	8563895	Flow cytometry analysis of CD4+CD29+ T cells, Cell viability assay, qPCR, Western blotting analysis, ELISA	Human
ADIPOQ	Adiponectin, C1Q and collagen domain containing	IBD, UC	9370	19714754, 18484673, 18383234, 16775490	ELISA, Fat-feeding protocol, RT-PCR, FACS Calibur cytofluorimeter	C57BL/6 Mice and Human
ABCB1	ATP binding Cassette subfamily B member 1	CD	5243	25346426, 16494108, 19685447	Genotyping, PCR, DHPLC	Human
NOX1	NADPH oxidase 1	IBD	27035	29091079	Genotyping, RT-qPCR	Human
C1GALT1C1	C1GALT1 specific chaperone 1	IBD	29071	27930307	Knockdown, PCR, Immunoblot, RNA expression chip	Mouse
SLC2A14	Solute carrier family 2 member 14	UC, CD	144195	28971850	SNP Selection, Genotyping	Human
CXCR4	C-X-C motif chemokine receptor 4	UC, CD	7852	24687413	PCR, SNP Genotyping	Human
ULK1	UNC-51 like autophagy activating kinase 1	CD	8408	21560199	(GWAS) SNPS was selected using information from the hapmap project, Genotyped using the sequenom mass array, PCR-RFLP	Human
IKBKG	Inhibitor of nuclear factor Kappa B kinase regulatory subunit gamma	CD	8517	15620648	Transfection, Immunoprecipitations, Western blotting, Antibodies, Plasmid and reagents, Mass Spectrometry, Reporter assays	Mouse and Human
CCL2	C-C motif chemokine ligand 2	UC	6347	18340639, 22763298, 23128233, 25620350	mRNA quantification, SYBR green PCR, Genotyping, PCR-RFLP	Human
IL6R	Interleukin 6 receptor	UC, CD	3570	10530495, 29775600	ELISA, RT-PCR, SNP analysis	Human
IL26	Interleukin 26	UC	55801	28852311	Immunohistochemistry, qPCR, Immunoblotting	Human	Up
RIPK3	Receptor interacting Serine/Threonine kinase 3	CD	11035	24322838	Immunoblot analysis, qPCR, Cell viability assay (MTT assay)	Human	Up
SLC22A5	Solute carrier family 22 member 5	UC	6584	21287663, 18274826	RNeasy mini kit (Qiagen), qPCR, Genotyping and haplotype analysis	Human	Down
IRF1	Interferon regulatory factor 1	CD	3659	17213842	(GWAS) Genotyping using sequenom mass array 7K system and MALDI-TOF MS analysis	Human
TYK2	Tyrosine kinase 2	UC, CD	7297	25744728, 19653082	(GWAS) Genotyping via qPCR, SNPS of Tyk2 gene were calculated, PCR-RFLP, Hapmap	Human
ICAM3	Intercellular adhesion molecule 3	IBD, UC	3385	27552332, 10931137	RT-qPCR, RT2 profiler human tight junction PCR array (Qiagen #Pahs-143Zc-12) for mRNA expression analysis of samples, Quantitative direct sandwich ELISA	Human	Up
IL27	Interleukin 27	UC, IBD	246778	19686419, 19915574, 27221243	(GWAS) RT-PCR, Genotype analysis of the IL-27 gene was performed by the single-base extension method, Immunohistochemistry, Rt-qPCR, Ficoll reagent	Human	Up
TNFRSF6B	TNF receptor superfamily member 6b	IBD, UC, CD	8771	18758464, 23965943, 20675196	(GWAS) Genome-wide SNP genotyping using illumina infinium humanhap550 beadchip technolo, ELISA, Gene expression analyses using affymetrix genechip Hg-U133 plus 2.0 arrays, Fluorescent-automated DNA sequencing using the Qiagen fast cycling PCR kit, Infrare	Human
CCR6	C-C motif chemokine receptor 6	IBD, UC	1235	29981781, 26536229, 22269120	Flow cytometry, ELISA, Histological examination, Immunostaining and in situ hybridization, Transcriptome analysis and RT-qPCR confirmation, Immunohistochemistry, Genotyping by PCR-RFLP	Human
LTA	Lymphotoxin alpha	CD, UC	4049	16246225, 8668926, 16931032	PCR-RFLP, DNA extraction, analysis of the TNF-_ gene polymorphism, ELISA	Human
LTB	Lymphotoxin beta	UC, CD	4050	8608636, 8612993	Indirect immunofluorescence technique, PCR-RFLP, DNA extraction, FITC conjugate	Human
ST2	Suppression of tumorigenicity 2	IBD, UC	9173	20385815, 21633527	Flow cytometry FACS, Immunohistochemistry, Immunohistochemical staining, RT-PCR, Western blot analysis, Sandwich and multiplex ELISA kits	SAMP and AKR Mice, Human
IL-36A	Interleukin 36 alpha	UC	27179	26813344	qPCR, Immunohistochemistry, Flow cytometry FACS	il36r_/_ mice
PTGER4	Prostaglandin E receptor 4	IBD, CD	5734	20222910, 20846217, 24793213	(GWAS) Selection of SNP, Genotyping using the sequenom mass array system, qPCR	Human
TNFSF11	TNF superfamily member 11	CD	8600	20848544, 17663430	Measurement of OPG By ELISA	Human
MUC1	Mucin 1, Cell surface associated	IBD, CD	4585	19025220, 28885228, 11559653	Immunohistochemistry, ELISA, Hematoxylin and Eosin staining, Mucosal gene expression Study, RT-qPCR, Expression quantitative trait loci (EQTL) mapping	Human
FADS2	Fatty acid sesaturase 2	CD	9415	24406470	(GWAS) SNP analysis	Human
TAGAP	T cell activation Rho GTPase activating protein	CD	117289	24582067, 23044675	Immunohistochemistry, qPCR, SNP genotyping	Human	Down
HMGB1	High mobility group box 1	IBD	3146	24983978, 22644337, 21788990, 9010268	Hematoxylin and Eosin techniques, qPCR, ELISA, Immunoblot analysis, Colonic myeloperoxidase assay	Mice C57BL6/J female mice
LCK	LCK Proto-oncogene, SRC family tyrosine kinase	IBD	3932	9329971	Immunohistochemistry, Western blot, Hematoxylin and Eosin techniques, Flow cytometry, FITC	Human
ZAP70	Zeta chain of T cell receptor associated protein kinase 70	UC, CD	7535	23406209	(GWAS) Mass spectrometric analysis (Iplex assay, Sequenom, San Diego, Ca) were used for genotyping on the sequenom mass array, Multiplex PCR, Spectrochip microarray, MALDI-TOF mass spectrometry	Human
CLEC2D	C-type lectin domain family 2 member D	IBD	29121	22664939	Genotyping for the SNPS was performed by PCR-RFLP	Human
RUNX3	RUNX family transcription factor 3	IBD	864	20887385, 18668679	(GWAS) Genotyping of all the SNP markers using Maldi_Tof MS sequenom�, qPCR, Immunohistochemical staining, Immunohistochemistry, mRNA by RT-PCR	Mice	Up
SERPINE1	Serpin family E member 1	UC, CD	5054	17111197, 24496159, 18844621, 12694086	Genotyping, Arms-PCR, Immunohistochemistry	Human
CD101	CD101 molecule	IBD	9398	26813346	Flow cytometry, Intracellular Ccytokine staining	Mice
NELL1	Neural EGFL like 1	CD, IBD	4745	17684544	SNP genotyping, RT-qPCR	Genome-wide case-control association
IBD6	Inflammatory bowel disease 6	IBD	50942	23071489	SNP-SNP interaction	Human cohort
IBD7	Inflammatory bowel disease7	IBD, CD	57042	23071489, 10814724, 20439597	SNP-SNP interaction, Serological testing	Human cohort
PRDM1	PR/SET domain 1	CD	639	23624108, 23028907	(GWAS) RT-qPCR, EQTL, Flow cytometry, SNP genotyping	Human
IL1R2	Interleukin 1 receptor type 2	UC	7850	26530134	RT-PCR, Immunofluorescence staining, ELISA, Flow cytometry	Human	Up
CDKAL1	CDK5 regulatory subunit associated protein 1 like 1	CD, UC	54901	19422935, 19174780, 19068216, 23128233, 20014019	(GWAS) SNP and genotyping, PCR-RFLP, MALDI-TOF Array, ELISA	Human
IRF8	Interferon regulatory factor 8	IBD, UC	3394	28166214, 22152681, 28700533	(GWAS) Genotyping array (Immunochip), qPCR System, SNP using Haploreg (V4.1), Flow cytometry and data analysis
DNMT3B	DNA methyltransferase 3 beta	IBD	1789	23128233, 23983426	(GWAS) SNP genotyping, Immunochip data, Network tools grail and dapple
MLN	Motilin	UC	4295	10439963, 18183601	Microsatellite markers, PCR, Linkage analysis, Genetic predisposition, RT_PCR, Immunohistochemistry, MPO assay, Storage phosphor autoradiography
LCN2	Lipocalin 2	UC	3934	23633156, 23668802, 22200942, 8675096, 10520846	ELISA, Westergreen method, Immunohistochemistry, MTT assay	Human and Mice	Up
REG3A	Regenerating family member 3 alpha	CD	5068	16847384, 15870231, 12792221, 12425429, 23519454	ELISA, Immunohistochemistry, RT-PCR, Microarray Gene expression analysis	Human	Up
CXCL2	C-X-C Motif chemokine ligand 2	UC	2920	19203362, 18835392	Luciferase assay, RT-qPCR, Dual immunohistochemistry and miRNA in situ hybridization, ELISA	Human	Up
REG1A	Regenerating family member 1 alpha	UC	5967	20065946, 15914572	RT-qPCR, Immunohistochemistry, Immunohistochemical staining, Western blot analysis, RNA interference, MTT and Tunel assays	Human	Up
RGS3	Regulator of G protein signaling 3	UC	5998	17262812	Immunohistochemistry, RT-qPCR, Significance analysis of microarrays (SAM) software was employed to analyze the gene expression data	Human	Up
MMP12	Matrix metallopeptidase 12	CD	4321	12842452, 19357521, 19201776, 17631138	High-density oligonucleotide array, In situ hybridization, Western blotting, RT-PCR, Immunoblotting, Wound healing scratch assay, Real-time MMP-12 activity assay, Flow cytometry, Collagen assay, Cell senescence detection, In situ collagen and a smooth mus	Human	Up
CLDN8	Claudin 8	CD, UC	9073	16822808, 16969477, 27029486	Immunohistochemistry and Confocal laser scanning microscopy, Freeze fracture electron microscopy, Western blot analysis, Dapi, Apoptotic ratio	Human	UP
SLC26A2	Solute carrier family 26 member 2	CD, UC	1836	24206446, 20848455, 24041540	RT_PCR, SNP genotyping	Human	Down
PCK1	Phosphoenolpyruvate carboxykinase 1	UC	5105	24436141, 30417021, 25991924	Total RNA isolation, qPCR, Microarray hybridization		Down
GUCA2A	Guanylate cyclase activator 2A	UC	2980	25979109	RT-qPCR, Western blot, SYBR primescript RT reagent Kits, Radioimmuno precipitation assay	Mice GC-C_/_	Down
AQP8	Aquaporin 8	UC	343	18467915, 17461471, 23595390	PCR, Immunohistochemistry, Affymetrix U133 gene chip expression analysis of RNA samples from IBD biopsy samples, SNP analysis, Microarray technology	Human	Down
SLC4A4	Solute carrier family 4 member 4	UC	8671	26278145, 17982090	qPCR, Microarray technology, DAVID, RT-qPCR, ELISA, Gene expression microarray	Human, Female Mice	Down
ABCA8	ATP binding cassette subfamily A member 8	UC	10351	18523026, 18776587	qPCR, Immunohistochemistry, In situ hybridisation, Microarray analysis, Taqman RT-PCR	Human	Down
SATB2	SATB homeobox 2	UC	23314	31318711	Tissue Microarray, Immunohistochemical analysis, Mutation and Fluorescence In situ hybridization		Down
CBR3-AS1	CBR3 antisense RNA 1	IBD	100506428	28153728	qPCR, Western blot, Chromatin immunoprecipitation (Chip) assay, Luciferase reporter assay, RNA-pull down assay	Human
ADAMDEC1	ADAM like decysin 1	UC	27299	17959550	Taqman qPCR, Prediction analysis of microarrays (Affymetrix Microarrays)	Human	Down
ANK3	Ankyrin 3	UC	288	26085826	Pediatric IBD microarray datasets, GeneSifter analysis, Ingenuity pathway analysis, TRANSFAC, Comparison between Pediatric IBD and Experimental colitis microarray data	Mice and Human	Down
NR1D2	Nuclear receptor subfamily 1 group D member 2	UC	9975	26172092, 26172092	(GWAS) Genome-wide cDNA microarray analysis, Circadian clock circuitry controls, Western blotting, ELISA	Human and Rev-erb__/_ mice	Down

图文展示

Gene symbol	Full name	Phenotypes	Entrez_id	PMID	Experimental evidences	Sample	Up OR Down
FRZB	Frizzled related protein	UC	2487	30270042, 23146664	Single cell RNA sequencing, Fluidigm C1, RT-PCR, Flow cytometry, Mass cytometry time-of-flight, Immunohistochemistry, Western blot analysis	Male mice	Down
HAPLN1	Hyaluronan and Proteoglycan link protein 1	UC	1404	23308121	(GWAS) SNP genotyping		Down
PRKACB	Protein kinase CAMP-activated catalytic subunit beta	UC	5567	17506908	Histopathological analysis, Genechip analysis, Hematoxylin and Eosin-staining, Microarray expression analysis	Mice	Down
SLC16A1	Solute carrier family 16 member 1	UC	6566	21987487	RT-qPCR, Gene expression	Human	Down
SELENBP1	Selenium binding protein 1	UC	8991	31019652	Cell viability assay, Cytokine Enzime-Linked Immunosorbent Assays, RNA isolation, qRT-PCR, Protein extraction, Western blotting, Luciferase Reporter Assay	Human, C57BL/6N- Selenbp1tm1b(KOMP)Wtsi/Mbp knockout mouse	Down
IL6ST	Interleukin 6 signal transducer	IBD	3572	15754404, 23128233, 9867112	DNA isolation and sequencing, PCR	Human
AGR2	Anterior gradient 2, Protein disulphide isomerase family member	IBD	10551	16222343	cDNA cloning, RT-PCR, Mutation detection, Dual luciferase reporter gene assay, SNP genotyping	Human, Mouse
HGF	Hepatocyte growth factor	IBD	3082	17317789, 15550554, 15906768, 10028449, 16261428	Flow cytometry analysis, ELISA, Immunostaining and morphometric analysis of intestinal microvasculature, in vitro tube formation assay, DSS-induced colitis, Western blot and immunoprecipitation analysis, Histopathological analysis	Mice
IL10RB	Interleukin 10 receptor subunit beta	PIBD, CD	3588	28267044, 16537974, 22550014, 24379584	Sanger Sequencing, Western blotting, RT-PCR	Human, Mice
ITGAX	Integrin subunit alpha X	UC	3687	12610322, 10935652, 9473377	Spectrofluorometric, Immunohistochemical staining	Human
ITGAL	Integrin subunit alpha L	IBD	3683	11862384, 14518246, 10935652, 19340879, 12139949	SNP genotyping, Immunohistochemistry, Flow cytometry analysis, Immunofluorescent staining	Human	UP
IL15RA	Interleukin 15 receptor subunit alpha	IBD	3601	23039249, 23128233, 15770395	Immunohistochemistry and Immunofluorescence, ELISA, Spectrophotometer (Nanodrop technologies), RT-qPCR	Human
IL12RB2	Interleukin 12 receptor subunit beta 2	CD	3595	22412388, 12492197, 11120856, 23128233	(GWAS) SNP genotyping, Flow cytometry, RT-PCR	Human
IL-37	Interleukin 37	IBD	27178	25214710, 23600829	Immunohistochemistry, qPCR, Western blot, ELISA		UP
OSM	Oncostatin M	IBD	5008	28368383, 23128233	(GWAS) Genome wide association studies, qPCR, Flow cytometry analysis of human intestinal mucosa, ELISA, RNA extraction cDNA synthesis, Immunohistochemistry, Total protein extraction and Immunoblot analysis	C57BL/6 Mice
PUS10	Pseudouridine synthase 10	UC, CD	150962	23128233, 19068216, 20228799, 21298027	(GWAS) SNP genotyping, RT-PCR, Luciferase activity, ER-stress reporter, Immunostaining and fluorescence microscopy	Human