IBD Integrated Resources Portal

轮播多图
Gene Symbol
Full name
Phenotypes
Entrez_id
PMID
Experimental evidences
Sample
Regulation
NKX2-3
NK2 homeobox 3
UC
159296
20957151, 21072187, 20165982
qPCR, RT-PCR, Genotyping, Western blot analysis, Ingenuity Pathway analysis, Microarray and data analysis, Immunofluorescence assay


NLRP3
NLR family pyrin domain containing 3
UC, CD
114548
19784369, 20385749, 30583612, 29102545, 28523573, 27966619, 20182451
SNP genotyping, qPCR, ELISA
Mice and Human

NOD2
Nucleotide binding oligomerization domain containing 2
IBD, CD
64127
25886994, 28099495, 25991924
miRNA microarray, RT-qPCR, SNP analysis
Human

NOD1
Nucleotide binding oligomerization domain containing 1
IBD
10392
21729873, 24155895
FACS analysis, RT-PCR and Microarray technology
Zebrafish

ATG16L1
Autophagy related 16 like 1
IBD, CD, UC
55054
28130498, 17435756, 20637199
Genotyping, IgA measurement, Western blotting, Histopathological analysis, RNA expression analysis, Immunofluorescence and Immunohistochemistry, RT-qPCR, SNP analysis, FACS and Microscopy
Human, Mice

TNF
Tumor necrosis factor
IBD, UC, CD
7124
22848538, 27895399, 12671897
Fish, qPCR, Histopathology, ELISA, Cytokine staining, RT-PCR and Western blotting
mice and Human

RIPK2
Receptor interacting serine/threonine kinase 2
IBD, CD
8767
26320862
Immunoblotting, qPCR and Flow cytometry
Human

TLR2
Toll like receptor 4
CD, UC, IBD
7097
28388655, 22434667, 19950179
Genotyping, SNP analysis, qPCR and FACS
Mice and Bacteria

TLR4
Toll like receptor 3
UC, CD, IBD
7099
29913461, 19664207, 17538633
qPCR and Western blotting, RFLP
Human

IL23R
Interleukin 23 receptor
IBD, UC, CD
149233
17447842, 17786191, 17030949
SNP analysis, FRET, qPCR, ELISA and FACS
Human, Mice

IRGM
Immunity related GTPase M
CD, UC, IBD
345611
20106866
Microarray expression analysis, qPCR, SNP analysis
Human

TLR3
Toll like receptor 3
IBD, UC
7098
28099447
1D SDS-PAGE, 2D Gel analysis and Gene expression


GAPDH
Glyceraldehyde-3-phosphate dehydrogenase
IBD, CD
2597
27812135, 19907652
(GWAS) RT-qPCR, Immunoblotting, ELISA, BRET analyses, Immunoprecipitation experiments, Yeast two-hybrid screening, SNP genotyping, siRNAs and NF-kappaB reporter assays, Western blotting with anti-MYC and anti-GFP antibodies


PTPN2
Protein tyrosine phosphatase non-receptor type 2
IBD, CD, UC
5771
22457781
(GWAS) PCR, DNA extraction, FRET and SNP genotyping, In silico analysis of transcription factor binding sites, Haplotype analysis
Human

MUC2
Mucin 2, oligomeric mucus/gel-forming
IBD, UC, CD
4583
27490946
Immunochip, High throughput sequencing


PIGR
Polymeric immunoglobulin receptor
IBD, CD, UC
5284
22491177
RT-qPCR, Tissue histology and Immunofluorescence microscopy

Down
BAHD1
Bromo adjacent homology domain containing 1
UC
22893
26183847
Immunohistochemistry, RT-qPCR, Western blot, ELISA, DSS induced colitic mice
Human, C57BL/6 Mice
Down
FFAR2
Free fatty acid receptor 2
IBD
2867
27667443
Immunohistochemistry, RT-qPCR, Western blotting

Down
FFAR3
Free fatty acid receptor 3
IBD
2865
27667443
Immunohistochemistry, RT-qPCR, Western blotting

Down
NCF2
Neutrophil cytosolic factor 2
UC
4688
21900546
Western blot, Cell culture and Immunoprecipitation, RT-PCR, Cell adhesion assay, NCF2 genotyping, SNP analysis and Genotyping, Subphenotype analysis, Apoptosis analysis

Up
RAC2
Rac family small GTPase 2
IBD, UC
5880
21900546
RT-PCR, Apoptosis analysis, SNP analysis and genotyping, Cell adhesion assay, Subphenotype analysis, Constructs, Western blot, Cell culture and Immunoprecipitation

Up
NCF4
Neutrophil cytosolic factor 4
CD
4689
21900546, 26289090
SNP genotyping and analysis, Subphenotype analysis, RT-PCR

Up
SEL1L
SEL1L adaptor subunit of ERAD E3 ubiquitin ligase
UC
6400
26551274
Western blot, Knockdown, RT-qPCR, Immunohistochemistry

Down
GLI1
GLI family zinc finger 1
UC
2735
19071955
RT-qPCR, SNP genotyping, Gene expression by microarray, NanoDrop ND-1000 spectrophotometer, Induction of DSS-induced colitis and Histological analysis, Immunohistochemistry, GLI1 protein mutagenesis, Luciferase assay and Immunofluorescence, Cytokine expre
Gli1+/lacZ Mice
Down
PTCH1
Patched 1
UC
5727
16733713
DNA extraction, SNP genotyping
Human
Down
IHH
Indian hedgehog signaling molecule
CD
3549
19071955, 20206176, 18479261
ELISA, DAVID, RT-qPCR
C57BL/6 mice
Up
AVIL
Advillin
IBD
10677
12034507
PCR, Mapping of AVIL by Fish, SNP analysis, Lightcycler analysis of AVIL mRNA expression, RT-PCR
Human

REL
REL Proto-Oncogene, NF-KB Subunit
UC
5966
21425313
(GWAS),Genotyping,and,SNP,selection,Allelic,association,analysis,Insilico,analysis,,GRAIL,analysis,eQTL,analysis,Principal,componenet,analysis
Human

ABCA1
ATP binding cassette subfamily A member 1
IBD
19
33609028
Western blot, Immunohistochemistry,Decreased mRNA expression of the LXR target genes ABCA1 and FAS was shown
IL-10-/- mice,Human

NR1H2
Nuclear receptor subfamily 1 group H member 2
UC,CD
7376
21245992
SNP genotyping, DNA extraction, Haplotype analysis
Human

PPARG
Peroxisome proliferator activated receptor gamma
UC,CD
5468
20591056, 26462850
RT-PCR, Gel electrophoresis,Total RNA extraction,qRT-PCR,Immunofluorescence,Immunohistochemistry,Bacterial culture,Cell culture
Human

TCF7L2
Transcription factor 7 like 2
UC
6934
19221600
SNP, Matrix assisted laser desorption/ionization time-of-flight (MALDI-TOF) based mass spectrometry (MS), RT-PCR, EMSA, Histological analysis, Promoter analysis, Sequencing of TCF-4 promoter and gene region, Genotyping TaqMan technology
Mice
Up
SLC22A4
Solute carrier family 22 member 4
CD
6583
19581171
SNP genotyping, PCR, Gene ontology
Human cohort study
Up
ICAM1
Intercellular adhesion molecule 1
IBD, UC, CD
3383
19212205, 18647548
Genotyping, SNP analysis, PCR, PCR-RFLP
Human

IL18RAP
Interleukin 18 receptor accessory protein
IBD, UC, CD
8807
19103669, 20176734, 18439550
SNP analysis, Western blotting
Human

JAK2
Janus kinase 2
IBD, UC, CD
3717
22065112
Genotyping,Real-time PCR
Mice, Human
Down
DEFA5
Defensin alpha 5
CD
1670
18305068
RT-PCR, Immunochemical staining

Low
APAF1
Apoptotic peptidase activating factor 1
UC
317
20113505
Immunohistochemistry,Immunoblotting,Gel electrophoresis,SDS-PAGE,Immunoprecipitation,


IL4
Interleukin 4
UC
3565
8964392
ELISA, RNA extraction and preparation of complementary DNA probes, Northern blot analysis


XBP1
X-box binding protein 1
IBD
7494
18775308
EM, qPCR, Microarray analysis, Immunohistochemistry, PAGE
Mice

CLEC7A
C-type lectin domain containing 7A
UC
64581
22674328
qPCR, Dapi, Immunofluorescent staining, Immunohistochemistry
Mice

ERBIN
Erbb2 interacting protein
CD
55914
19266573
Genotyping, RT-PCR, Western blot, Immunoprecipitation and Immunoblotting
Human

SLC15A1
Solute carrier family 15 member 1
IBD
6564
19462432
Genotyping, Dual-Luciferase Reporter Assay, MALDI-TOF mass spectrometry
Male mice

MUC19
Mucin 19, Oligomeric
IBD, UC, CD
283463
23128233, 19714762, 21152001
(GWAS) SNP genotyping, MALDI TOF
Human

CYLD
CYLD lysine 63 deubiquitinase
IBD, CD
1540
24092863
SNP analysis, siRNA transfection, PCR, Western blotting, Confocal microscopy


NOS2
Nitric oxide synthase 2
IBD, UC, CD
4843
24430113
SNP analysis and Genotyping, Immunohistochemistry


MTOR
Mechanistic target of rapamycin kinase
UC
2475
30132862
FITC, Myeloid-Derived Suppressor cells (MDSCs) differentiation assay, Flow cytometry analysis, DSS-induced experimental colitis in mice, ROS detection, Real-time PCR
Mice

ECM1
Extracellular matrix protein 1
UC
1893
28699600
RFLP-PCR, Genotyping
Human

BCL2
BCL2 apoptosis regulator
IBD, CD
596
30228896
FITC, FACS, RT-PCR, Fcal-ELISA


BAX
BCL2 associated X, Apoptosis regulator
UC, IBD, CD
581
30228896
FITC, FACS, RT-PCR, Fcal- ELISA


TLR10
Toll like receptor 10
IBD, CD
81793
21716313
qPCR, FRET, Genotyping, Taqman assay


TLR8
Toll like receptor 8
IBD
51311
18942751, 26486764
Genotyping, SNP analysis
Human cohort study

TLR7
Toll like receptor 7
IBD
51284
27084119
DSS treatment, qPCR, Microarray analysis, BlastN, Genotyping, Immunohistochemistry
Mice

TLR9
Toll like receptor 9
CD
54106
22893852, 17914947
(GWAS) Genotyping, RT-PCR, PCR-RFLP
Mice
Low
IL17A
Interleukin 17A
CD, UC
3605
26072972
qPCR, Immunohistochemistry, ELISA, FACS Caliber Flow cytometry
Human
Up
IL23A
Interleukin 23 subunit alpha
IBD, CD, UC
51561
20228197
qPCR, Cytokine ELISA, Chip, Flow cytometry
Mice
Down
MYD88
MYD88 innate immune signal transduction adaptor
IBD, CD, UC
4615
20433840
qPCR, Dapi, Immunofluorescent staining, Flow cytometry
Mice

CARD9
Caspase recruitment domain family member 9
IBD, UC, CD
64170
26488816, 29073062, 27158904
qPCR, In vivo injection of heat-killed C.albicans in PBS, Ex vivo splenic stimulations and intracellular phosphoprotein staining, FACS, Bead based-ELISA, Western blotting, Immunoprecipitation
Mice Strain -Trim62 and WT

CD4
CD4 molecule
IBD, UC, CD
920
26589955
Immunofluorescence, Suppression assay, FACS Aria cell sorter
Human

IL6
Interleukin 6
IBD, UC, CD
3569
19550417, 18799360, 22539302
Immunofluorescence microscopy, Western blotting, Flow cytometry, ELISA assay, qPCR, Genotype, MALDI-TOF/MS
Human cohort study

TLR5
Toll like receptor 5
CD
7100
16439468
SDS-PAGE immunoblotting, ELISA, Genotyping, Human cytokine array, Infection of adenoviruses, Immunofluorescence staining, PCR, Immunoprecipitation assay, Luciferase reporter assay
Human, Mice

DAP
Death associated protein
UC
1611
21297633
(GWAS) SNP analysis, In silico analysis
Meta analysis, Human cohort

PTPN22
Protein tyrosine phosphatase non-receptor type 22
UC
26191
23456301, 15986374, 16185327, 16391555, 16164701
Genotyping, RFLP-PCR, Serum level determination by immuno-nephelometry with Behring Nephelometer Analyzer II (BNII), mRNA expression analysis
Human

LRRK2
Leucine rich repeat kinase 2
CD
120892
29321258, 22430149
(GWAS) Exome sequencing, Human exome bead chip genotyping, Immunochip-base study, Immunoblot, Western blot, SDS-PAGE, Flow cytometry, GTP hydrolysis assay, TLC
Human, Mice










图文展示


Gene symbol
Full name
Phenotypes
Entrez_id
PMID
Experimental evidences
Sample
Up OR Down
TNFSF15
TNF superfamily member 15
CD
9966
19124533, 24835165, 24783249, 18422820, 18338776, 25028192
SNP genotyping, Kaplan meier method, ELISA
Human cohort study

IL12B
Interleukin 12B
IBD
3593
22479607, 23573954, 15483662
(GWAS) SNP analysis, PCR, FRET, Insilico analysis
Human cohort study

IBD5
Inflammatory bowel disease 5
CD
50941
18521914, 16642031
Fast qPCR, SNP analysis, Gene-gene Interaction analysis
Human cohort study
Up
IL18
Interleukin 18
IBD, UC, CD
3606
26229413, 12572878, 14738461
Meta-analysis by database searches, Cytokine assays by Sandwich ELISA, Histological Examination, Flow cytometry, qPCR
Human cohort, Mice

NLRP6
NLR family pyrin domain containing 6
CD
171389
21593405, 20403135
RT-PCR, DSS-treated mice, ELISA, Immunohistochemistry, Microarray and Gene ontology analysis
Mice

NLRP1
NLR family pyrin domain containing 1
IBD, CD
22861
25725098
ELISA, Generation of chimeric mice by bone marrow reconstitution, Histopathology evaluation, Polyp analysis, Human metadata analysis
Mice Nlrp1b_/_ and Asc_/_

TLR1
Toll like receptor 1
CD
7096
17595233
RFLP-PCR genotype, Serological analyses, ELISA, Multivariate genotype-phenotype-serotype associations in crohn'S disease
Human cohort

TLR6
Toll like receptor 6
CD
10333
24670426
DSS-induced colitis model, Immunoblot, Immunofluorescence staining, qPCR, Ex vivo T-cell Stimulation, PBMC culture, FACS canto II Flow cytometer, Fcap array
Human, Mice

TNFAIP3
TNF alpha induced protein 3
UC, CD
7128
25956706
Multivariate analysis of biomarker expression in colonic mucosa, Taqman gold RT-PCR, mRNA transcripts analyzed by RT-qPCR
Human

CD14
CD14 molecule
CD, UC
929
15764151, 15655821
RFLP-PCR, Genotyping
Human cohort

NLRP12
NLR family pyrin domain containing 12
IBD, CD
91662
22094258, 31169706, 20403135
In situ intestinal proliferation assay, In vitro signaling assays, Multiplex ELISA, qPCR, Induction of DSS induced colitis, Histopathological analysis, Bone marrow chimeras, Isolation of lamina propria cells, NF-KappaB activity assay
Mice
Down
CIITA
Class II MHC transactivator
IBD, CD
4261
17043423
DSS-induced colitis, Oxazolone-induced colitis, Cytokine ELISA, Histopathological analysis
Mice C57BL/6 (B6)

XIAP
X-Linked inhibitor of apoptosis
IBD, CD
331
28404814
Antibodies and Western blotting, NOD signaling defects and cell death susceptibility, CRISPR/SPCAS9 knockout of XIAP and stable cell line generation, qPCR, TNF and LPS cytotoxicity assays, Propidium iodide influx assays, Acquisition of patient PBMCs and F
XIAP/ mice

APC
APC regulator of WNT signaling pathway
UC, IBD
324
9744312, 17192888
PCR, PAGE, Ileorectal anastomosis (IRA), in vitro synthe-sized protein (IVSP)


DLG5
Discs large MAGUK scaffold protein 5
CD, IBD, UC
9231
16494592, 15107852
Genotyping for DLG5 variants, PCR, FRET probes, Measurement of gastro-duodenal and intestinal permeability, Genotyping for Card15 variants, SNPs genotyping analysis, Microsatellite typing
Human

DMBT1
Deleted in malignant brain tumors 1
CD, IBD
1755
17983803, 24223725
siRNA transfection, qPCR, Western blot, Electrophoretic mobility shift, Luciferase assays, RT-qPCR, Immunohistochemistry, mRNA In situ hybridization, Haplotype association
Human, Mice
DMBT SNPs; Up
TNFRSF17
TNF receptor superfamily member 17
IBD
608
16318629
SNP genotyping, Tailed primer PCR, Microsatellite markers analysis
Human

KLF6
Kruppel like factor 6
IBD
1316
26838049
Western blot, RT-PCR, Immunofluorescent confocal staining
Mice
Up
FUT3
Fucosyl transferase 3 (Lewis Blood Group)
UC
2525
26766790
SNP genotyping, Immunohistochemistry
Human
FUT3 polymorphisms overrepresented in UC patients vs controls
FUT2
Fucosyl transferase 2
CD, IBD
2524
24720527, 20570966, 24828903, 22068912, 23075394, 23002346
Genotype analysis By multiplex SNAP assay of Abi, Multiplex PCR, SNP tested in replication of FUT loci by taqman minor groove binder chemistry, Taqman SNP genotyping assays
Human cohort

IBD2
Inflammatory bowel disease 2
IBD, UC
3378
12439891
Genotyping, PCR, Electrophoretic mobility shift assay (EMSA), Criterion blotter, Chemiluminescent nucleic acid detection module (Pierce Biotechnology, Rockford, Il, Usa)
Human

IRF5
Interferon regulatory factor 5
IBD, UC, CD
3663
20127100, 22257839, 3809990, 25564941
Bisulfite pyrosequencing, CpG density calculation
Human

NLRX1
NLR family member X1
IBD
79671
29535731
Experimental model of colitis, Histopathology, Olympus microscope, qPCR, RNA sequencing
Mice C57BL/6 wild-type (WT), Nlrx1_/_, Nlrx1fl/fl, and Nlrx1fl/fl, Villin Cre+ mice
Up
BTNL2
Butyrophilin like 2
UC, IBD, CD
56244
17610417, 19659809, 17237401, 25671699
Direct genome sequencing, PCR-RFLP, Association study of HLA_DRB1
Human cohort

MST1
Macrophage stimulating 1
IBD, UC, CD
4485
19657358, 19079170, 22087277, 18438406, 21931648
(GWAS) Genotyping by Taqman assays, Fast qPCR, SNP analysis, SNP Selection from hapmap database, Mass spectrometry analysis, Allelic tests, RT-PCR, SNP genotyping with infinium I assay (Illumina), Plink, MALDI-TOF mass array platform
Human cohort

CUL2
Cullin 2
IBD, CD
8453
23639549, 30228239
(GWAS) SNP Test, RT-PCR, Gtex SNP(rs12248333), IBD-associated SNP (rs34779708), EQTL analysis, Haplotype analyses, Selection analyses, Credible set analysis, E. Histolytica culture, Cre-luciferase reporter assays, Crem expression In Q223R mice, Caspase-3
SV129/Bl6.CREM_/_ Mice

FOXP3
Forkhead box P3
IBD, UC
50943
27178149, 28337881
(GWAS) qPCR, mRNA expression, Measurement of serum retinol levels by HPLC, Immunohistochemistry, Histopathology
Human

DUOX2
Dual oxidase 2
IBD, CD, UC
50506
26301257, 25003194, 17454855
Epifluorescence light microscope (Leica microsystems, Buffalo Grove, Il), Protein isolation, Western blotting, Ros assays, Flow cytometry, Immunofluorescence, Virulence assay, Cytoscape.V3.0.2, Immunohistochemistry, Olympus Bx51 light microscope, RT-PCR
Huamn

IL2
Interleukin 2
UC, IBD, CD
3558
21648020, 19201773, 19471255, 8100205, 12591057
SNP analysis, Taqman technology for genotyping, Mass spectrometric analysis, ELISA, ADCC assay, Imminofluorescence staining, Flow cytometry
Human

MYC
MYC proto-oncogene, BHLH transcription factor
IBD, CD
4609
1612482
RNA preparation, Slot blot hybridization, Northern hybridization, in vitro translation, Immunohistochemical studies, expression of C-Myc proto-oncogene
Human

IL22
Interleukin 22
UC, CD
50616
16537974, 26994530
RT-PCR, qPCR, Signal-transduction experiments, Gel electrophoresis, Immunoblotting, ELISA, Wounding assay, Cell proliferation assay, Apoptosis assays, DSS induced colitis model
Mice, Human
Up
HDAC6
Histone deacetylase 6
IBD
10013
27827303
Light microscope, DSS induced colitis, Histology, Mucin staining, Esterase staining, Gut cell isolation, Flow cytometry, RT-PCR analysis, Isolation of human polymorphonuclear leukocytes, HPLC, MS
Female C57BL/6 mice

DEFB1
Defensin beta 1
CD
1672
17921115, 22522766
RT-PCR, Fluorescent microscope, qPCR, DEFB1 SNP genotyping
Human cohort

BIRC3
Baculoviral IAP repeat containing 3
UC
330
23388545
RNA extraction, Microarray analysis, Gene filtering and sample classification were performed using Dchip software, qPCR, Immunohistochemistry
Human

RNF186
Ring finger protein 186
UC
54546
24068945, 27503255
(GWAS) DNA preparation and pooling, PCR, Target selection and design, Sequencing and variant discovery, Genotyping, Validation and follow-up genotyping using sequenom mass array Iplex200, Network analyses, Exome sequencing, MALDI-TOF, Immunofluorescence,
Human cohort, Mice

CRP
C-Reactive protein
pIBD, CD, UC
1401
16497618, 25636121, 18566104
Genotyping, PCR-RFLP, SNP selection, DNA extraction from venous whole blood by salting out technique, Response to antiTNF_ monoclonal antibody
Human cohort

IL2RA
Interleukin 2 receptor subunit alpha
UC, IBD
3559
23972291, 19471255
Genotyping using primer extension chemistry and Mass spectrometric analysis(Iplex assay, Sequenom, San Diego, Ca), Multiplex PCR, MALDI-TOF Mass spectrometry, Genotyping by Taqman assays from applied biosystems


CD40
CD40 molecule
IBD, CD, UC
958
15591506, 10566730, 16149136
Elucidation of the sequence of rat CD40 mRNA, Immunohistochemistry, Fluorescein (FITC), Immunofluorescence microscopy, Transfection of antisense oligonucleotides(ODNS) in vitro, Western blot analysis, RT-PCR analysis, Colitis induction, Flow cytometric an
Rat

MEFV
MEFV innate Immuity regulator, Pyrin
CD
4210
22810105, 15667491
(GWAS) Genotyping for The MEFV variants, PCR
Human

AIM2
Absent in melanoma 2
UC
9447
26313692, 26655906
Histopathological examination, Affymetrix microarrays, RT-qPCR, RNA isolation, RNA quantity and quality assessed using nanodrop Nd-1000 spectrophotometer, Microarray gene expression data analysed using limma, Immunohistochemistry, Western blot analysis, P
Human Cohort, Mice
Down
BIRC2
Baculoviral IAP repeat containing 2
UC
329
30005591, 28422189
(GWAS) Geo data collection and preprocessing, Network clustering by DPCLUSO, DEGs

UP
CCL5
C-C motif chemokine ligand 5
IBD, UC
6352
19639558, 11872088
qPCR, Immunocytochemistry, Proteomics, Histopathology, Microarray analysis, (LC/MS/MS), Microbial analysis by denaturing gradient gel electrophoresis (DGGE), Sequencing of DGGE bands
Mice IL10-KO and wildtype (WT)
Down
ATG7
Autophagy related 7
UC
10533
30669622, 19139628
(GWAS) Lysozyme staining, The role of the autophagy pathway in paneth cell abnormalities
Mice

AKT1
AKT Serine/Threonine kinase 1
IBD
207
28659381
Induction of colitis, Haematoxylin and Eosin staining, Immunohistochemistry, HCT-8 and Hela cells were used for transfection, Western blot, qPCR, Rt_ Profiler PCR array data analysis web-based tool (Qiagen, Sa Biosciences, Usa), Immunoprecipitation, ELIS
Female C57BL/6 mice
Down
MPO
Myeloperoxidase
UC, IBD, CD
4353
18810778
Calprotectin ELISA, Radioimmunoassay, Histopathology


PSTPIP1
Proline-serine-threonine phosphatase interacting protein 1
IBD
9051
19731031
EDTA, DHPLC, PCR, PSTPIP1 Microsatellite identification and typing, Genomatix suite used for promoter sequences
Human




Gene Symbol
Full name
Phenotypes
Entrez_id
PMID
Experimental evidences
Sample
Up OR Down
PGLYRP1
Peptidoglycan recognition protein 1
IBD
8993
20709292, 23840689
Western blotting, PGRP-Deficient mice have higher susceptibility to DSS induced colitis, Flow cytometry, RT-qPCR, Cell isolation, Culture, Cytokine production, Amino acid sequence homology in mammalian PGLYRPS in the regions of non-synonymous polymorphism
Mice   BALB/c

CD80
CD80 molecule
IBD, UC, CD
941
25595911, 14530338
Flow cytometry, Histopathology, Immunohistochemistry, RT-qPCR, Immunoassay, Induction of colitis and treatments, Cytokine ELISA
C57Bl6/J Mice, BALB/c mice

ICOSLG
Inducible T cell costimulator ligand
IBD, CD
23308
24837102, 20176734, 21072187
(GWAS) FACS, ELISA, Transfection of small interfering RNAs (siRNAs), Western blot, qPCR, Genotyping and association tests
Human, Mice

ITLN1
Intelectin 1
CD
55600
18587394
(GWAS) Haplotype patterns, Hapmap SNPs, EQTL analysis, SNP analysis


IL9
Interleukin 9
UC, IBD
3578
28652656, 25838986, 26377844
Flow cytometry based method utilizing magnetic microspheres, High-sensitive C-reactive protein (HSCRP), Fluorescence based assay, qPCR, Western blot analysis, RT-PCR, Immunofluorescence microscopy, TNBS colitis model, Immunofluorescence staining, ELISA
BALB/c and IL-9 deficient mice
up-regulation
IFNG
Interferon gamma
UC
3458
20848535, 25171510
(GWAS) IFN-assay By ELISA, Pyrosequencing, Hot-start PCR, Isolation of T cells
Human

IL13RA2
Interleukin 13 receptor subunit alpha 2
IBD
3598
22741617, 28612843, 20014020, 30663072
PCR-RFLP, SNP analysis, Immunohistochemistry
Balb/c mice, Human

ZNF365
Zinc finger protein 365
CD
22891
21257989, 20222910, 25489960
RNase minikit (Qiagen, Valencia, Ca), SNP selection and genotype, RT-PCR, Illumina total prep RNA amplification kit (protocol from Ambion, Austin, Tx), Nanodrop spectrophotometer, qPCR
Human

LAMB1
Laminin subunit beta 1
UC, IBD
3912
19915572
(GWAS) Picogreen assay, Sequenom Iplex assay, GWA genotyping, Replication genotyping, SNP analysis


CD68
CD68 molecule
CD
968
26802079
Bradford assay, Immunostaining, Immunohistochemistry, Immunofluorescence and Goblet cell count used as a secondary antibody, Western blot, FITC, RT-qPCR, Chromatin immunoprecipitation (Chip) assay
Human

HNRNPA1
Heterogeneous nuclear ribonucleoprotein A1
CD
3178
19349988
Immunoblot analysis, Chip, RT-PCR, DNA affinity binding assay, Retroviral and Lentiviral packaging and macrophage transduction, Immuno-precipitation, Nanoflol Liquid chromatographytandem MS analysis, ELISA
Mice

KCNN4
Potassium calcium-activated channel subfamily N member 4
CD
3783
20407432
RT-PCR, qPCR, Immunohistochemistry, MALDI-TOF mass spectrometer, SNP identification and genotyping, Sequenom mass-array assay
Mice, Human

SIGIRR
Single Ig and TIR domain containing
UC
59307
21077278, 20130217
Immunoprecipitation (Chip) assays, Human IL-8 enzyme immune assay (EIA), FITC, qPCR, Immunohistochemistry assays, Flow cytometry, RNA interference, Transient transfection and NF-KappaB, Luciferase assay, Chromatin immunoprecipitation (Chip) assay, Gel shi
Mice
Down
ORMDL3
ORMDL Sphingolipid biosynthesis regulator 3
CD
94103
21072187, 27932194, 28333149, 20228799
(GWAS) SNP genotyping assay(Taqman), qPCR, SNP analysis, DSS-induced colitis in mice
Human, Mice
Up
TOLLIP
Toll interacting protein
UC, CD
54472
18985539, 26462859
Luciferase assay, Electrophoretic mobility shift assay, Immunoblotting, qPCR, Immunofluorescence microscopy, Immuno-electron microscopy (Em), Indirect Immunofluorescence microscopy, ELISA
Female BALB/c mice
Up
OLFM4
Olfactomedin 4
IBD
10562
20941359, 30137272
(GWAS) Microarray analysis of RNA expression, RT-PCR, Data analysis, NCBI gene expression omnibus (Geo), qPCR, Library preparation and Next-generation sequencing with the SeqCap Epi CpGiant enrichment kit
Human
Up
CCL21
C-C motif chemokine ligand 21
UC, CD
6366
24841666, 16118351, 26520877
Immunohistochemistry, DSS induced colitis, RT-PCR, Western blotting, Dapi, Immunohistochemistry staining in the intestinal mucosa of mice
Balb/c mice
Up
ATG16L2
Autophagy related 16 Like 2
CD
89849
27611316, 23850713
(GWAS) Genotyped using taqman SNP genotyping assay, Relationship between genotypes and phenotypes, qPCR, Bioinformatics analysis, mRNA expression of ATG16L2, Imputation
Human

SLC25A20
Solute carrier family 25 member 20
IBD, UC
788
26874350
Bacterial preparations, RNA isolation, Microarrays, qPCR, RT-PCR
Human
Up
SLC15A4
Solute carrier family 15 member 4
IBD, UC
121260
21277849
Immunohistochemical staining, Quantification of cytokines using a bead cytometric bead array mouse inflammation kit, Assay for cathepsin activities, DSS induced colitis model, Bone marrow transplantation, Stimulation of mice with NOD ligands, Flow cytomet
Mice   C57BL/6 Slc15a4-Deficient mice

NLRP7
NLR family Pyrin domain containing 7
UC, CD
199713
29211899
Whole-exome sequencing (Wes), Mapping, Pathogenicity prediction, PCR, Sanger Sequencing confirmation of NLRP7 variants, Genotyping
Human

GSDMB
Gasdermin B
UC, CD
55876
28154144, 26484354
Cloning, Expression, Protein purification, Crystallization, Data collection, Structure determination, Protein lipid overlay assay, in vitro caspase cleavage assay, SDS-PAGE, Nanodrop Nd-1000, RT-PCR, Gene expression analysis and Data preprocessing, Genoty
Human

EGFR
Epidermal growth factor receptor
UC
1956
24391216, 29904166
DSS-induced colitis, Western blot analysis, Multi-color flow cytometry, Myeloperoxidase (MPO) assay, Immunophenotyping, Colonic macrophage isolation, Isolation of spleen lymphocytes, qPCR, ELISA, Luciferase activity, SDS-PAGE, Immunohistochemistry, FITC,
Egfrfl/flLysM-Cre mice and UC Human

MAPK3
Mitogen-activated protein kinase 3
IBD, CD
5595
26636514
Bioinformatic analysis, Affymetrix microarray data, Identification of differentially expressed genes, Gene ontology and pathway enrichment analysis of DEGs using Kegg, Go and David, PPI Network and Module analysis, RT-qPCR analysis
Human, Rats

MAP3K8
Mitogen-activated protein kinase 8
IBD, UC
1326
26215868
Transfection of small interfering RNAs (SiRNAs) and Plasmids, Protein expression analysis, mRNA expression analysis, qPCR, RT-PCR, Genotyping, Primary human myeloid cells from IBD, Flow cytometry
Human

RNF31
Ring finger protein 31
IBD, UC
55072
31132297
Human cohort and microarray analysis, Evaluation of mouse colitis, Immunohistochemistry and Immunofluorescent hybridisation, Reagents and antibodies
Male BALB/c mice
Up
IL31RA
Interleukin 31 receptor A
IBD, UC
133396
17449633
RT-PCR, qPCR, SYBR green PCR kit, Signal transduction experiments, Gel electrophoresis, Immunoblotting, Immunofluorescence staining, ELISA, Cell proliferation and cell restitution assays, Murine cytomegalovirus infection in vivo
C57BL/6 wild type Mice
Up
IL21
Interleukin 21
UC
59067
21648020
SNP genotyping, qPCR
Human

IL15
Interleukin 15
UC, CD
3600
10725717, 26964669
FITC or Pe-conjugated anti-CD3, Flow cytometric analysis, Immunohistochemistry, IL-15 production by LPMC, T cell activation by IL-15, Measurement of cytokines, ELISA, In situ expression of IL-15 In intestinal tissue
Human

ATG4D
Autophagy related 4D cysteine peptidase
CD
84971
22261526
(GWAS) Genotyping, RFLP
Human

LACC1
Laccase domain containing 1
CD
144811
27959965, 27098602
Western blot, Transfection, Bradford colorimetric assay, SDS PAGE, Immunofluorescence staining, Antibodies used for immunofluorescence and western blot, Laccase assay, qPCR, FACS flow cytometry, In silico analyses, SNP genotype
Human
Up
FASLG
Fas ligand
CD, IBD, UC
356
22860894, 26374830, 9771405
Genotyping, Immunohistochemistry, Assessment of immunohistochemical staining, RT-PCR, In situ hybridization, Flow cytometry
Human, Mouse

IL7
Interleukin 7
IBD, CD
3574
23057802, 18097039
Induction of colitis and antibody treatments, Pathology and Immunohistochemistry, Serum cytokines and anti-Hb antibodies, Quantitative RT-PCR analyses, ELISA, Flow cytometry
Mice Mdr1a_/_, Rag2_/_ mice, C57BL/6-Ly5.2 mice, C57BL/6-Ly5.1 mice and C57BL/6-Ly5.2-RAG-2-deficient (RAG-2/) mice

ATG4A
Autophagy related 4A cysteine peptidase
CD
115201
22261526, 21122541
m
Human

EGF
Epidermal growth factor
IBD, CD
1950
20969541
Sandwich ELISA
Human

CD44
CD44 molecule (Indian Blood Group)
IBD, UC
960
9863483, 10378517
Antibody characterization ELISA, Immunohistological detection of CD44 v6 expression in ulcerative colitis and crohnS disease, Hewlett-packard ELISA reader, Determination of soluble CD44 v6 96 well immunoassay plates (Immunoplate I), Staining with antibod
Mice BALB/c

IL13
Interleukin 13
IBD, UC, CD
3596
22741617, 23746199
Genotyping determined by PCR-RFLP


CD19
CD19 molecule
CD
930
25914477
Demditec Epstein-Barr Virus VCA IgG antibody ELISA test kit (Demeditec diagnostics Gmbh, Kiel, Germany), Anti-EBV IgG antibodies
Human

CDKN2A
Cyclin dependent kinase inhibitor 2A
UC
1029
29063720
(GWAS) Genotyping using illumina omniexpress, Omni express exome and Omni1-quad arrays, 7900Ht fast qPCR system, UCSC genome bioinformatics and Genecards were utilized, SNP was performed using the encode, Genotype tissue expression (Gtex)29
Human
Up
THY1
Thy-1 cell surface antigen
UC
7070
22826509, 22202458
(GWAS) Qiaamp tissue DNA preparation kit (Qiagen), Three-layer genome wide scans for differential gene expression, Discordant for UC, Expression profiling (mRNA, Layer I), Human methylation27 beadchips (Iscan System, Illumina), Validation of differential
Human
Up
IFNA1
Interferon alpha 1
CD
3439
7861027
Dot blot analysis
Human
Up
CD86
CD86 molecule
IBD, CD, UC
942
18069758, 9438323
PCR, Immunocytometry, Flow cytometric analysis
Human
Up
CSF2
Colony stimulating factor 2
UC, CD
1437
29434451, 19230854
ELISA
Human
Up
TP53
Tumor protein P53
UC, CD
7157
20110711, 17676397
SNP genotyping, Immunohistochemistry staining
Human
Up
CEACAM6
CEA cell adhesion molecule 6
CD
4680
19737864
Histological analyses, Confocal microscopy
Mice
Up
CXCL8
C-X-C motif chemokine ligand 8
CD
3576
15214047
Immunohistochemical staining, ELISA
Human
Down






Gene Symbol
Full name
Phenotypes
Entrez_id
PMID
Experimental evidences
Sample
Up   OR Down
ATG5
Autophagy related 5
CD
9474
27096233
SNP genotyping
Human
Down
NFKB1
Nuclear factor Kappa B subunit 1
CD
4790
18092345, 17049230, 24945726
Chemiluminescence, Western blot, SNP genotyping, RT-qPCR, Immunohistochemical staining
Human
Up
LRP6
LDL receptor related protein 6
CD
4040
22393312
SNP genotyping, qPCR, Immunohistochemistry
Human
Down
STAT1
Signal transducer and activator of transcription 1
UC
6772
27220814, 23128233, 12171960
Flow cytometry FACS aria cytometer, LPMCs were cultured in 200 ml complete medium in 96-well plates, qPCR, Multiplex ELISA, Staining of cells for phenotyping and counting, Plasmacytoid dendritic cell isolation kit, PBMCs were isolated by density-gradient
Human
Up
IL10
Interleukin 10
CD
3586
16043989, 29023267, 24128120, 24216686
Abi Prism 3100 automatic sequencer (applied biosystems, Foster City, Calif), Genemapper V3.0 (applied biosystems), SNP genotyping, qPCR, FACS canto II Flow cytometer, Analyzed using flowjo, Ingenuity pathway analysis, RNA in situ hybridization, Immunochem
Mice(Il10-/- and Il10rb-/- mice)

DEFA6
Defensin alpha 6
CD
1671
26891258, 19549264, 18305068
qPCR, Luciferase assays, Chromatin Immunoprecipitation assay, Immunohistochemistry, RT-PCR validation of selected Transcripts, Variability in gene expression profiles of two independent sets of UC RNA samples, Chromosomal locations of differentially expre
Human
Up
TREM1
Triggering receptor expressed on myeloid cells 1
IBD
54210
22721842, 21910175, 21763322, 18975078
ELISA, Westergren method, Trem-1 SNP and genotyping
Human
Up
CX3CL1
C-X3-C motif chemokine ligand 1
IBD
6376
17241867, 17285595
Taqman technology, qPCR, Stimulation of himec with cytokines, Leukocytes and CD40L, Flow cytometric using a coulter epics Xl flow cytometer (Beckman Coulter Inc., Fullerton, Ca), Characterization of himec and PBMC, Immunohistochemistry, Western blotting,
Human
Up
HORMAD2
HORMA domain containing 2
CD
150280
19915574
(GWAS) Selection of SNPS genotyping
Human

IBD4
Inflammatory bowel disease 4
CD
50608
10747815
Genotyping by Abi prism linkage mapping set version 2, IBD susceptibility genes
Human

MOCOS
Molybdenum cofactor sulfurase
CD
55034
19500084
Known SNPS in (Xdh, Mocos and Aox1) selected using the applied biosystems, Taqman SNP genotyping assays, qPCR, Sequencing by Beckman Coulter dye terminator sequencing kit
Human

MIF
Macrophage migration inhibitory factor
IBD, UC, CD
4282
24687413, 17206642, 18452219, 18023095, 12109441
RFLP-PCR, Genotyping, ELISA, RT-qPCR, Spectrophotometry, Western blot analysis, Cytokine assays, Flow cytometry
Human

MME
Membrane metallo-endopeptidase
IBD
4311
23827863
RNA isolation and qPCR, High-capacity cDNA reverse transcription kit, DNA isolation and genotyping
Human

TNFRSF25
TNF receptor superfamily member 25
IBD, CD
8718
27001939, 26072972
FACS calibur flow cytometer (Bd) using cell quest analysis software (Bd), RNA isolation, qPCR, Primer-blast software, ELISA
Human
Up
VWF
Von willebrand factor
IBD, UC
7450
14966927
ELISA, Radioimmunoassay, Platelet count (Pc), Immunoassay
Human

TFF3
Trefoil factor 3
UC
7033
15932366
Sigmoidoscopic and Histological scoring
Human
Down
CCR7
C-C motif chemokine receptor 7
CD
1236
16118351
Immunohistochemistry, RT-PCR analysis, Immunostaining
Human cohort

AHR
Aryl hydrocarbon receptor
IBD, CD
196
20878756, 26802080
Fluorescent immunohistochemistry, Histological staining, RNA isolation kit, RT-PCR, SYBR green qPCR supermix, Bead based immunoassay, Proliferating cell nuclear antigen assay
Male C57BL/6 and AhR _/_ mice
Up
HMOX1
Heme oxygenase 1
UC, IBD
3162
18460015, 16804400, 20509889
Genotype assessment, Taqman qPCR, Microsatellite instability, Western blot, Immunohistochemical staining, Genotyping
Human
Up
ITGB2
Integrin subunit Beta 2
IBD, UC, CD
3689
12139949, 17109102, 9438323
Immunofluorescent labeling, FITC, Flow cytometry analysis
Human

POU5F1
POU class 5 homeobox 1
IBD, UC
5460
25664710
DNA isolation and genotyping, Spectrophotometer, Illumina veracode microarray Chip, SNP genotypes


CSF1
Colony stimulating factor 1
IBD, UC
1435
11889070, 28273887
Radioimmunoassays and Immunoassays, Immunohistochemistry, ELISA
Human
Up
CCR5
C-C motif chemokine receptor 5 (Gene/Pseudogene)
IBD, CD, UC
1234
28273887
Western blot analysis, Flow cytometry, ELISA
Human
Up
VEGFA
Vascular endothelial growth factor A
IBD, UC, CD
7422
27270504, 16911686, 15113369, 10389696, 17879276, 29556626, 25076849, 17188371, 16954806
Immunohistochemistry and Image analyses, Immunostaining, ELISA, Immunoassay, Immunohistochemical studies of VEGF, SNPs analysis

Down
CFLAR
CASP8 and FADD like apoptosis regulator
UC, CD
8837
23371318, 16472594
Immunohistochemistry, IEC purity determination by   RT-PCR, RT-qPCR, Antibodies, Immunoblotting, Chemiluminescence
Human
Up
IL17F
Interleukin 17F
UC, IBD
112744
17828618, 19263269, 18022867, 26558270
Genotyping of polymorphisms by PCR-SSCP method, One-tube multiplex PCR, PCR-RFLP


PTEN
Phosphatase and Tensin homolog
UC
5728
20067111, 23516074
Immunohistochemistry, Immunohistochemical detection, Western blot analysis, Tunel assay
female Balb/c mice

F9
Coagulation factor IX
CD
2158
29945876
DSS-Induced colitis model and tissue preparation, Immunoblotting, Myeloperoxidase activity assay, CXCL1 ELISA
C57BL/6J WT control mice (F9+/+), F9_/_ mice

IGF1
Insulin like growth factor 1
IBD, CD
3479
21287667, 14691340, 11914023, 19751734
Serum assays, ELISA method, Western immunoblotting, qPCR, Immunostaining
Human cohort

CASP3
Caspase 3
IBD, CD
836
24553140, 15479684
EBV-transformed B-cell lines, Taqman SNP genotyping assay, Immortalized macrophage progenitors, Cellular ATG16L1 cleavage assays, Image-based flow cytometry, Western blots and Caspase assays
Mice

S100A9
S100 calcium binding protein A9
IBD1, CD, UC
6280
25781366, 29115397, 28138990, 28735301
Measurement of fecal calprotectin by quantitative ELISA, Immunochromatographic technology, Quantitative fecal immunochemical test, Immunohistochemistry, Immunocytochemistry, Relationships between post operative endoscopic recurrence, Fcal and HSCRP levels
Human

FCGR3B
Fc fragment of IgG receptor IIIB
IBD, UC
2215
23917248, 23128233
(GWAS) Multiplex PCR, SNP genotype
Human

CD2
CD2 molecule
CD, UC
914
7680015
Immunohistological labeling, in vitro cell aggregation, Effect of blocking antibodies against surface adhesion molecules
Human

SRC
SRC Proto-oncogene, Non-receptor tyrosine kinase
UC
6714
8674385
Isolation of colonic epithelial cells and purification of RNA, Probes, Electroelution and Chromatography on an Elutip-D column, Northern Blotting of RNA, Slotblotting, Radioactively labeled probes
Human
Down
LEP
Leptin
IBD, UC, CD
3952
17700265, 18383234, 9586759, 15784704, 15012781, 18849144
ELISA, Radioimmunoassay of stored serum, Erythrocyte sedimentation rate, BMI, Serum albumin and C_reactive protein concentrations and white blood cell counts were determined
Human

PI3
Peptidase inhibitor 3
UC, IBD
5266
29084078, 17200145
RNA extraction and RT-qPCR assays, Immunohistochemical analysis, ELISA
Human

CA2
Carbonic anhydrase 2
UC
760
11956656, 27742763
ELISA
Human

BCAR1
BCAR1 scaffold protein, Cas family member
CD, PIBD
9564
23665963
(GWAS) ELISA, SNP analysis


NLRP10
NLR family Pyrin domain containing 10
CD
338322
20403135, 25153506
(GWAS) Haplotype tagging SNPS, Sub-phenotype analysis


TIRAP
TIR domain containing adaptor protein
IBD
114609
17538633, 29441063
Genotyping using the sequenom mass array system (all genotypes on collections IBD-1 To -5 were generated), Determining haplotypes


PSMD4
Proteasome 26S subunit, Non-ATPase 4
IBD, UC
5710
14621278, 10807895
Immunohistochemical staining, A lowering in the inflammatory blood parameters ESR, CRP andorosomucoid was demonstrated, Antisecretory Factor, Preparation of AF and test of AF potency, Histopathological examination of rectal biopsies
Human

FCGR3A
Fc fragment of IgG receptor III A
IBD, CD, UC
2214
23917248, 29333082, 23128233, 21091999
ELISA, PCR-RFLP, SNP genotyping, PCR-based real-time invader assay


ITGAM
Integrin subunit alpha M
CD, UC, IBD
3684
9473377, 9355789
SNP genotyping, Flow cytometry triple fluorescence analysis


PIK3CA
Phosphatidylinositol-4, 5-bisphosphate 3-kinase catalytic subunit alpha
UC
5290
21442372
ELISA, DSS-induced colitis, Stained with Hematoxylin and Eosin, Immunohistochemistry
Female Balb/c mice

HLA-DRA
Major histocompatibility complex, Class II, DR alpha
UC, CD
3105
23128233, 19122664, 12073071
(GWAS) EQTL SNPS, Network tools grail and dapple, SNP analysis, All downstream genotyping done by SNPlex and Taqman technologies


VIM
Vimentin
CD, IBD
7431
21699783, 18626977, 2124983
Co-Immunoprecipitation, Genotyping by Taqman, SNP analysis, Immunofluorescence, Luciferase assay, Western blots, Hematoxylin and Eosin staining, Immunohistochemistry
Human

CD28
CD28 molecule
IBD
940
14518246
Immunohistochemistry
Human

CDKN1A
Cyclin dependent kinase inhibitor 1A
IBD, UC, CD
1026
18507080, 24760188, 15060836
RT-qPCR, Illumina mRNA microarray platform was used for mRNA microarray assays, Transfection of miR-Mimic and Inhibitors, Western blotting, Cell cycle analysis by flow cytometry, Luciferase reporter assays
Human
Up
CCDC122
Coiled-coil domain containing 122
CD
160857
22984114
(GWAS) Genotyping using high-throughput sequenom mass array platform, SNP analysis
Human

FAS
Fas cell surface death receptor
UC, CD, IBD
355
15637757, 16049281, 16856205
PCR, SNP analysis, DHPLC, Haematoxylin and Eosin staining, Immunoreactivity scores, Immunohistochemistry, Flow cytometry
Human
Up
FASN
Fatty acid synthase
IBD, UC
2194
16753600
Immunohistochemistry
Human
Up
JUN
Jun Proto-oncogene, AP-1 transcription factor subunit
IBD
3725
8674385
Cytoplasmic, Nuclear, Tumor suppressor genes proto-oncogene probes were used, Electro-elution and Chromatography on an Elutip-D column, Slot blotting and Northern blotting of RNA
Human

DEFB4A
Defensin beta 4A
UC, IBD, CD
1673
19617917, 17387677, 18346318, 12519391
ELISA, Immunoelectron microscopy, Immunohistochemistry, qPCR, ImmunoFlow cytometry
Human

IRAK3
Interleukin 1 receptor associated kinase 3
IBD, UC
11213
17558906
Genotyping and SNP selection, Microsatellite marker PCR
Human





图文展示
Gene Symbol
Full name
Phenotypes
Entrez_id
PMID
Experimental evidences
Sample
Up OR Down
ITGB1
Integrin subunit beta 1
IBD, UC
3688
8563895
Flow cytometry analysis of CD4+CD29+ T cells, Cell viability assay, qPCR, Western blotting analysis, ELISA
Human

ADIPOQ
Adiponectin, C1Q and collagen domain containing
IBD, UC
9370
19714754, 18484673, 18383234, 16775490
ELISA, Fat-feeding protocol, RT-PCR, FACS Calibur cytofluorimeter
C57BL/6 Mice and Human

ABCB1
ATP binding Cassette subfamily B member 1
CD
5243
25346426, 16494108, 19685447
Genotyping, PCR, DHPLC
Human

NOX1
NADPH oxidase 1
IBD
27035
29091079
Genotyping, RT-qPCR
Human

C1GALT1C1
C1GALT1 specific chaperone 1
IBD
29071
27930307
Knockdown, PCR, Immunoblot, RNA expression chip
Mouse

SLC2A14
Solute carrier family 2 member 14
UC, CD
144195
28971850
SNP Selection, Genotyping
Human

CXCR4
C-X-C motif chemokine receptor 4
UC, CD
7852
24687413
PCR,   SNP Genotyping
Human

ULK1
UNC-51 like autophagy activating kinase 1
CD
8408
21560199
(GWAS) SNPS was selected using information from the hapmap project, Genotyped using the sequenom mass array, PCR-RFLP
Human

IKBKG
Inhibitor of nuclear factor Kappa B kinase regulatory subunit gamma
CD
8517
15620648
Transfection, Immunoprecipitations, Western blotting, Antibodies, Plasmid and reagents, Mass Spectrometry, Reporter assays
Mouse and Human

CCL2
C-C motif chemokine ligand 2
UC
6347
18340639, 22763298, 23128233, 25620350
mRNA quantification, SYBR green PCR, Genotyping, PCR-RFLP
Human

IL6R
Interleukin 6 receptor
UC, CD
3570
10530495, 29775600
ELISA, RT-PCR, SNP analysis
Human

IL26
Interleukin 26
UC
55801
28852311
Immunohistochemistry, qPCR, Immunoblotting
Human
Up
RIPK3
Receptor interacting Serine/Threonine kinase 3
CD
11035
24322838
Immunoblot analysis, qPCR, Cell viability assay (MTT assay)
Human
Up
SLC22A5
Solute carrier family 22 member 5
UC
6584
21287663, 18274826
RNeasy mini kit (Qiagen), qPCR, Genotyping and haplotype analysis
Human
Down
IRF1
Interferon regulatory factor 1
CD
3659
17213842
(GWAS) Genotyping using sequenom mass array 7K system and MALDI-TOF MS analysis
Human

TYK2
Tyrosine kinase 2
UC, CD
7297
25744728, 19653082
(GWAS) Genotyping via qPCR, SNPS of Tyk2 gene were calculated, PCR-RFLP, Hapmap
Human

ICAM3
Intercellular adhesion molecule 3
IBD, UC
3385
27552332, 10931137
RT-qPCR, RT2 profiler human tight junction PCR array (Qiagen #Pahs-143Zc-12) for mRNA expression analysis of samples, Quantitative direct sandwich ELISA
Human
Up
IL27
Interleukin 27
UC, IBD
246778
19686419, 19915574, 27221243
(GWAS) RT-PCR, Genotype analysis of the IL-27 gene was performed by the single-base extension method, Immunohistochemistry, Rt-qPCR, Ficoll reagent
Human
Up
TNFRSF6B
TNF receptor superfamily member 6b
IBD, UC, CD
8771
18758464, 23965943, 20675196
(GWAS) Genome-wide SNP genotyping using illumina infinium humanhap550 beadchip technolo, ELISA, Gene expression analyses using affymetrix genechip Hg-U133 plus 2.0 arrays, Fluorescent-automated DNA sequencing using the Qiagen fast cycling PCR kit, Infrare
Human

CCR6
C-C motif chemokine receptor 6
IBD, UC
1235
29981781, 26536229, 22269120
Flow cytometry, ELISA, Histological examination, Immunostaining and in situ hybridization, Transcriptome analysis and RT-qPCR confirmation, Immunohistochemistry, Genotyping by PCR-RFLP
Human

LTA
Lymphotoxin alpha
CD, UC
4049
16246225, 8668926, 16931032
PCR-RFLP, DNA extraction, analysis of the TNF-_ gene polymorphism, ELISA
Human

LTB
Lymphotoxin beta
UC, CD
4050
8608636, 8612993
Indirect immunofluorescence technique, PCR-RFLP, DNA extraction, FITC conjugate
Human

ST2
Suppression of tumorigenicity 2
IBD, UC
9173
20385815, 21633527
Flow cytometry FACS, Immunohistochemistry, Immunohistochemical staining, RT-PCR, Western blot analysis, Sandwich and multiplex ELISA kits
SAMP and AKR Mice, Human

IL-36A
Interleukin 36 alpha
UC
27179
26813344
qPCR, Immunohistochemistry, Flow cytometry FACS
il36r_/_ mice

PTGER4
Prostaglandin E receptor 4
IBD, CD
5734
20222910, 20846217, 24793213
(GWAS) Selection of SNP, Genotyping using the sequenom mass array system, qPCR
Human

TNFSF11
TNF superfamily member 11
CD
8600
20848544, 17663430
Measurement of OPG By ELISA
Human

MUC1
Mucin 1, Cell surface associated
IBD, CD
4585
19025220, 28885228, 11559653
Immunohistochemistry, ELISA, Hematoxylin and Eosin staining, Mucosal gene expression Study, RT-qPCR, Expression quantitative trait loci (EQTL) mapping
Human

FADS2
Fatty acid sesaturase 2
CD
9415
24406470
(GWAS) SNP analysis
Human

TAGAP
T cell activation Rho GTPase activating protein
CD
117289
24582067, 23044675
Immunohistochemistry, qPCR, SNP genotyping
Human
Down
HMGB1
High mobility group box 1
IBD
3146
24983978, 22644337, 21788990, 9010268
Hematoxylin and Eosin techniques, qPCR, ELISA, Immunoblot analysis, Colonic myeloperoxidase assay
Mice C57BL6/J female mice

LCK
LCK Proto-oncogene, SRC family tyrosine kinase
IBD
3932
9329971
Immunohistochemistry, Western blot, Hematoxylin and Eosin techniques, Flow cytometry,   FITC
Human

ZAP70
Zeta chain of T cell receptor associated protein kinase 70
UC, CD
7535
23406209
(GWAS) Mass spectrometric analysis (Iplex assay, Sequenom, San Diego, Ca) were used for genotyping on the sequenom mass array, Multiplex PCR, Spectrochip microarray, MALDI-TOF mass spectrometry
Human

CLEC2D
C-type lectin domain family 2 member D
IBD
29121
22664939
Genotyping for the SNPS was performed by PCR-RFLP
Human

RUNX3
RUNX family transcription factor 3
IBD
864
20887385, 18668679
(GWAS) Genotyping of all the SNP markers using Maldi_Tof MS sequenom, qPCR, Immunohistochemical staining, Immunohistochemistry, mRNA by RT-PCR
Mice
Up
SERPINE1
Serpin family E member 1
UC, CD
5054
17111197, 24496159, 18844621, 12694086
Genotyping, Arms-PCR, Immunohistochemistry
Human

CD101
CD101 molecule
IBD
9398
26813346
Flow cytometry, Intracellular Ccytokine staining
Mice

NELL1
Neural EGFL like 1
CD, IBD
4745
17684544
SNP genotyping, RT-qPCR
Genome-wide case-control association

IBD6
Inflammatory bowel disease 6
IBD
50942
23071489
SNP-SNP interaction
Human cohort

IBD7
Inflammatory bowel disease7
IBD, CD
57042
23071489, 10814724, 20439597
SNP-SNP interaction, Serological testing
Human cohort

PRDM1
PR/SET domain 1
CD
639
23624108, 23028907
(GWAS) RT-qPCR, EQTL, Flow cytometry, SNP genotyping
Human

IL1R2
Interleukin 1 receptor type 2
UC
7850
26530134
RT-PCR, Immunofluorescence staining, ELISA, Flow cytometry
Human
Up
CDKAL1
CDK5 regulatory subunit associated protein 1 like 1
CD, UC
54901
19422935, 19174780, 19068216, 23128233, 20014019
(GWAS) SNP and genotyping, PCR-RFLP, MALDI-TOF Array, ELISA
Human

IRF8
Interferon regulatory factor 8
IBD, UC
3394
28166214, 22152681, 28700533
(GWAS) Genotyping array (Immunochip), qPCR System, SNP using Haploreg (V4.1), Flow cytometry and data analysis


DNMT3B
DNA methyltransferase 3 beta
IBD
1789
23128233, 23983426
(GWAS) SNP genotyping, Immunochip data, Network tools grail and dapple


MLN
Motilin
UC
4295
10439963, 18183601
Microsatellite markers, PCR, Linkage analysis, Genetic predisposition, RT_PCR, Immunohistochemistry, MPO assay, Storage phosphor autoradiography


LCN2
Lipocalin 2
UC
3934
23633156, 23668802, 22200942, 8675096, 10520846
ELISA, Westergreen method, Immunohistochemistry, MTT assay
Human and Mice
Up
REG3A
Regenerating family member 3 alpha
CD
5068
16847384, 15870231, 12792221, 12425429, 23519454
ELISA, Immunohistochemistry, RT-PCR, Microarray Gene expression analysis
Human
Up
CXCL2
C-X-C Motif chemokine ligand 2
UC
2920
19203362, 18835392
Luciferase assay, RT-qPCR, Dual immunohistochemistry and miRNA in situ hybridization, ELISA
Human
Up
REG1A
Regenerating family member 1 alpha
UC
5967
20065946, 15914572
RT-qPCR, Immunohistochemistry, Immunohistochemical staining, Western blot analysis, RNA interference, MTT and Tunel assays
Human
Up
RGS3
Regulator of G protein signaling 3
UC
5998
17262812
Immunohistochemistry, RT-qPCR, Significance analysis of microarrays (SAM) software was employed to analyze the gene expression data
Human
Up
MMP12
Matrix metallopeptidase 12
CD
4321
12842452, 19357521, 19201776, 17631138
High-density oligonucleotide array, In situ hybridization, Western blotting, RT-PCR, Immunoblotting, Wound healing scratch assay, Real-time MMP-12 activity assay, Flow cytometry, Collagen assay, Cell senescence detection, In situ collagen and a smooth mus
Human
Up
CLDN8
Claudin 8
CD, UC
9073
16822808, 16969477, 27029486
Immunohistochemistry and Confocal laser scanning microscopy, Freeze fracture electron microscopy, Western blot analysis, Dapi, Apoptotic ratio
Human
UP
SLC26A2
Solute carrier family 26 member 2
CD, UC
1836
24206446, 20848455, 24041540
RT_PCR, SNP genotyping
Human
Down
PCK1
Phosphoenolpyruvate carboxykinase 1
UC
5105
24436141, 30417021, 25991924
Total RNA isolation, qPCR, Microarray hybridization

Down
GUCA2A
Guanylate cyclase activator 2A
UC
2980
25979109
RT-qPCR, Western blot, SYBR primescript RT reagent Kits, Radioimmuno precipitation assay
Mice GC-C_/_
Down
AQP8
Aquaporin 8
UC
343
18467915, 17461471, 23595390
PCR, Immunohistochemistry, Affymetrix U133 gene chip expression analysis of RNA samples from IBD biopsy samples, SNP analysis, Microarray technology
Human
Down
SLC4A4
Solute carrier family 4 member 4
UC
8671
26278145, 17982090
qPCR, Microarray technology, DAVID, RT-qPCR, ELISA, Gene expression microarray
Human, Female Mice
Down
ABCA8
ATP binding cassette subfamily A member 8
UC
10351
18523026, 18776587
qPCR, Immunohistochemistry, In situ hybridisation, Microarray analysis, Taqman RT-PCR
Human
Down
SATB2
SATB homeobox 2
UC
23314
31318711
Tissue Microarray, Immunohistochemical analysis, Mutation and Fluorescence In situ hybridization

Down
CBR3-AS1
CBR3 antisense RNA 1
IBD
100506428
28153728
qPCR, Western blot, Chromatin immunoprecipitation (Chip) assay, Luciferase reporter assay, RNA-pull down assay
Human

ADAMDEC1
ADAM like decysin 1
UC
27299
17959550
Taqman qPCR, Prediction analysis of microarrays (Affymetrix Microarrays)
Human
Down
ANK3
Ankyrin 3
UC
288
26085826
Pediatric IBD microarray datasets, GeneSifter analysis, Ingenuity pathway analysis, TRANSFAC, Comparison between Pediatric IBD and Experimental colitis microarray data
Mice and Human
Down
NR1D2
Nuclear receptor subfamily 1 group D member 2
UC
9975
26172092, 26172092
(GWAS) Genome-wide cDNA microarray analysis, Circadian clock circuitry controls, Western blotting, ELISA
Human and Rev-erb__/_ mice
Down





图文展示
Gene symbol
Full name
Phenotypes
Entrez_id
PMID
Experimental evidences
Sample
Up OR Down
FRZB
Frizzled related protein
UC
2487
30270042, 23146664
Single cell RNA sequencing, Fluidigm C1, RT-PCR, Flow cytometry, Mass cytometry time-of-flight, Immunohistochemistry, Western blot analysis
Male mice
Down
HAPLN1
Hyaluronan and Proteoglycan link protein 1
UC
1404
23308121
(GWAS) SNP genotyping

Down
PRKACB
Protein kinase CAMP-activated catalytic subunit beta
UC
5567
17506908
Histopathological analysis, Genechip analysis, Hematoxylin and Eosin-staining, Microarray expression analysis
Mice
Down
SLC16A1
Solute carrier family 16 member 1
UC
6566
21987487
RT-qPCR, Gene expression
Human
Down
SELENBP1
Selenium binding protein 1
UC
8991
31019652
Cell viability assay, Cytokine Enzime-Linked Immunosorbent Assays, RNA isolation, qRT-PCR, Protein extraction, Western blotting, Luciferase Reporter Assay
Human, C57BL/6N- Selenbp1tm1b(KOMP)Wtsi/Mbp knockout mouse
Down
IL6ST
Interleukin 6 signal transducer
IBD
3572
15754404, 23128233, 9867112
DNA isolation and sequencing, PCR
Human

AGR2
Anterior gradient 2, Protein disulphide isomerase family member
IBD
10551
16222343
cDNA cloning, RT-PCR, Mutation detection, Dual luciferase reporter gene assay, SNP genotyping
Human, Mouse

HGF
Hepatocyte growth factor
IBD
3082
17317789, 15550554, 15906768, 10028449, 16261428
Flow cytometry analysis, ELISA, Immunostaining and morphometric analysis of intestinal microvasculature, in vitro tube formation assay, DSS-induced   colitis, Western blot and immunoprecipitation analysis, Histopathological analysis
Mice

IL10RB
Interleukin 10 receptor subunit beta
PIBD, CD
3588
28267044, 16537974, 22550014, 24379584
Sanger Sequencing, Western blotting, RT-PCR
Human, Mice

ITGAX
Integrin subunit alpha X
UC
3687
12610322, 10935652, 9473377
Spectrofluorometric, Immunohistochemical staining
Human

ITGAL
Integrin subunit alpha L
IBD
3683
11862384, 14518246, 10935652, 19340879, 12139949
SNP genotyping, Immunohistochemistry, Flow cytometry analysis, Immunofluorescent staining
Human
UP
IL15RA
Interleukin 15 receptor subunit alpha
IBD
3601
23039249, 23128233, 15770395
Immunohistochemistry and Immunofluorescence, ELISA, Spectrophotometer (Nanodrop technologies), RT-qPCR
Human

IL12RB2
Interleukin 12 receptor subunit beta 2
CD
3595
22412388, 12492197, 11120856, 23128233
(GWAS) SNP genotyping, Flow cytometry, RT-PCR
Human

IL-37
Interleukin 37
IBD
27178
25214710, 23600829
Immunohistochemistry, qPCR, Western blot, ELISA

UP
OSM
Oncostatin M
IBD
5008
28368383, 23128233
(GWAS) Genome wide association studies, qPCR, Flow cytometry analysis of human intestinal mucosa, ELISA, RNA extraction cDNA synthesis, Immunohistochemistry, Total protein extraction and Immunoblot analysis
C57BL/6 Mice

PUS10
Pseudouridine synthase 10
UC, CD
150962
23128233, 19068216, 20228799, 21298027
(GWAS) SNP genotyping, RT-PCR, Luciferase activity, ER-stress reporter, Immunostaining and fluorescence microscopy
Human